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Gene sequence analysis of toxins from the spider Phoneutria nigriventer revealed an intronless feature
Paiva, Ana Luiza Bittencourt; Matavel, Alessandra; Silva, Bruno César Souza; Guerra-Duarte, Clara; Diniz, Marcelo Ribeiro Vasconcelos.
Affiliation
  • Paiva, Ana Luiza Bittencourt; Fundação Ezequiel Dias. Belo Horizonte. BR
  • Matavel, Alessandra; Fundação Ezequiel Dias. Belo Horizonte. BR
  • Silva, Bruno César Souza; Universidade Federal de Minas Gerais - UFMG. Belo Horizonte. BR
  • Guerra-Duarte, Clara; Fundação Ezequiel Dias. Belo Horizonte. BR
  • Diniz, Marcelo Ribeiro Vasconcelos; Fundação Ezequiel Dias. Belo Horizonte. BR
J. venom. anim. toxins incl. trop. dis ; J. venom. anim. toxins incl. trop. dis;26: e20190075, 2020. tab, graf, ilus
Article de En | LILACS, VETINDEX | ID: biblio-1101266
Bibliothèque responsable: BR68.1
ABSTRACT
Phoneutria nigriventer spider venom contains several cysteine-rich peptide toxins that act on different ion channels. Despite extensive studies on its venom and description of cDNA sequences of several of its toxin precursors, the gene structure of these toxins remains unknown.

Methods:

Genomic regions encoding the precursors of three previously characterized P. nigriventer toxins - PnTx1, PnTx2-5 and PnTx4(5-5) - were amplified by PCR using specific primers. PCR fragments were cloned and sequenced. Obtained sequences were compared with their corresponding cDNA sequences.

Results:

The size of PCR fragments obtained and sequences corresponding to genomic regions encoding for the toxin precursors matched their cDNA sequences.

Conclusions:

Despite a few nucleotide substitutions in the genomic regions encoding for the toxin precursors when compared with cDNA sequences, the results of the present work indicate that P. nigriventer toxins do not contain introns in their genes sequences.(AU)
Sujet(s)
Mots clés

Texte intégral: 1 Indice: LILACS Sujet Principal: Venins d'araignée / Introns / Réaction de polymérisation en chaîne / Analyse de séquence / Cystéine / Nucléotides Limites du sujet: Animals langue: En Texte intégral: J. venom. anim. toxins incl. trop. dis Thème du journal: TOXICOLOGIA Année: 2020 Type: Article

Texte intégral: 1 Indice: LILACS Sujet Principal: Venins d'araignée / Introns / Réaction de polymérisation en chaîne / Analyse de séquence / Cystéine / Nucléotides Limites du sujet: Animals langue: En Texte intégral: J. venom. anim. toxins incl. trop. dis Thème du journal: TOXICOLOGIA Année: 2020 Type: Article