Detection of Drug Resistant Mycobacterium Tuberculosis Strains Using Kit SIRE Nitratase®: a Multicenter Study
Braz. arch. biol. technol
; 63: e20190179, 2020. tab, graf
Article
de En
| LILACS
| ID: biblio-1132181
Bibliothèque responsable:
BR1.1
ABSTRACT
Abstract (1) Background:
The Commercial Kit SIRE Nitratase® PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2)Methods:
To determine its accuracy, the proportion methods in Lowenstein Jensen medium or the BACTECTMMGITTM960 system was used as a gold standard. (3)Results:
The study revealed that the respective accuracies of the kit with 190 M. tuberculosis clinical isolates, using the proportion methods in Lowenstein Jensen medium or BACTECTMMGITTM960 system as a gold standard, were 93.9% and 94.6%, 96.9% and 94.6%, 98.0% and 97.8%, and 98.0% and 98.9%, for streptomycin, isoniazid, rifampicin, and ethambutol, respectively. (4)Conclusion:
Thus, the kit can rapidly screen resistance to streptomycin, isoniazid, rifampicin, and ethambutol. Additionally, it does not require sophisticated equipment; hence, it can be easily used in the laboratories of low and middle income countries.Mots clés
Texte intégral:
1
Indice:
LILACS
Sujet Principal:
Tuberculose multirésistante
/
Antibiotiques antituberculeux
/
Mycobacterium tuberculosis
Type d'étude:
Clinical_trials
/
Diagnostic_studies
Limites du sujet:
Humans
langue:
En
Texte intégral:
Braz. arch. biol. technol
Thème du journal:
BIOLOGIA
Année:
2020
Type:
Article