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Risk Factors for Early Childhood Caries Based on Identification of Veillonella spp. Using RT-PCR
Setiawan, Asty Samiaty; Darwita, Risqa Rina; Susilawati, Sri; Maharani, Diah Ayu; Djais, Ariadna Adisattya.
Affiliation
  • Setiawan, Asty Samiaty; University of Padjadjaran. Faculty of Dentistry. Department of Community Dental Health. Bandung. ID
  • Darwita, Risqa Rina; University of Indonesia. Faculty of Dentistry. Department of Dental Health and Preventive Dentistry. Jakarta. ID
  • Susilawati, Sri; University of Padjadjaran. Faculty of Dentistry. Department of Community Dental Health. Bandung. ID
  • Maharani, Diah Ayu; University of Indonesia. Faculty of Dentistry. Department of Dental Health and Preventive Dentistry. Jakarta. ID
  • Djais, Ariadna Adisattya; University of Indonesia. Faculty of Dentistry. Department of Oral Biology. Jakarta. ID
Article de En | LILACS, BBO | ID: biblio-1135530
Bibliothèque responsable: BR1264.1
ABSTRACT
Abstract

Objective:

To identify the occurrence of Veillonella spp. in children using real-time PCR (RT-PCR) and to determine its role as a risk factor for ECC in children aged 2-3 years. Material and

Methods:

A cross-sectional survey was conducted and samples from 87 children aged 2-3 years, who lived in selected villages in the Bandung City area, Indonesia, were collected. Examination for dental caries was performed using standard checks for decay, missing, and filled surfaces (dfms), and saliva samples were taken. Microbiological examination was performed using RT-PCR with primers consisting of one primary set for Veillonella spp. and one universal primary set for 16S rDNA. We performed statistical testing using the Mann Whitney rank-sum test.

Results:

A total of 87 children were sampled, and an ECC prevalence of 71.3% was found, with a mean dmfs of 7.1 (± 9.1). The proportion of Veillonella spp. in caries-free children was 2.13 ± 2.30, while in children with ECC, it was 3.29 ± 6.83.

Conclusion:

The proportion of Veillonella spp. in children with ECC was higher than in caries-free children; therefore, Veillonella spp. may be a risk factor for ECC.
Sujet(s)
Mots clés

Texte intégral: 1 Indice: LILACS Sujet Principal: Veillonella / Réaction de polymérisation en chaîne / Facteurs de risque / Caries dentaires / Bactéries à Gram négatif Type d'étude: Diagnostic_studies / Etiology_studies / Observational_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Limites du sujet: Child, preschool / Female / Humans / Male Pays comme sujet: Asia langue: En Texte intégral: Pesqui. bras. odontopediatria clín. integr Thème du journal: ODONTOLOGIA Année: 2020 Type: Article

Texte intégral: 1 Indice: LILACS Sujet Principal: Veillonella / Réaction de polymérisation en chaîne / Facteurs de risque / Caries dentaires / Bactéries à Gram négatif Type d'étude: Diagnostic_studies / Etiology_studies / Observational_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Limites du sujet: Child, preschool / Female / Humans / Male Pays comme sujet: Asia langue: En Texte intégral: Pesqui. bras. odontopediatria clín. integr Thème du journal: ODONTOLOGIA Année: 2020 Type: Article