Risk Factors for Early Childhood Caries Based on Identification of Veillonella spp. Using RT-PCR
Pesqui. bras. odontopediatria clín. integr
; 20: e5701, 2020. tab
Article
de En
| LILACS, BBO
| ID: biblio-1135530
Bibliothèque responsable:
BR1264.1
ABSTRACT
Abstract Objective:
To identify the occurrence of Veillonella spp. in children using real-time PCR (RT-PCR) and to determine its role as a risk factor for ECC in children aged 2-3 years. Material andMethods:
A cross-sectional survey was conducted and samples from 87 children aged 2-3 years, who lived in selected villages in the Bandung City area, Indonesia, were collected. Examination for dental caries was performed using standard checks for decay, missing, and filled surfaces (dfms), and saliva samples were taken. Microbiological examination was performed using RT-PCR with primers consisting of one primary set for Veillonella spp. and one universal primary set for 16S rDNA. We performed statistical testing using the Mann Whitney rank-sum test.Results:
A total of 87 children were sampled, and an ECC prevalence of 71.3% was found, with a mean dmfs of 7.1 (± 9.1). The proportion of Veillonella spp. in caries-free children was 2.13 ± 2.30, while in children with ECC, it was 3.29 ± 6.83.Conclusion:
The proportion of Veillonella spp. in children with ECC was higher than in caries-free children; therefore, Veillonella spp. may be a risk factor for ECC.Mots clés
Texte intégral:
1
Indice:
LILACS
Sujet Principal:
Veillonella
/
Réaction de polymérisation en chaîne
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Facteurs de risque
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Caries dentaires
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Bactéries à Gram négatif
Type d'étude:
Diagnostic_studies
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Etiology_studies
/
Observational_studies
/
Prevalence_studies
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Prognostic_studies
/
Risk_factors_studies
Limites du sujet:
Child, preschool
/
Female
/
Humans
/
Male
Pays comme sujet:
Asia
langue:
En
Texte intégral:
Pesqui. bras. odontopediatria clín. integr
Thème du journal:
ODONTOLOGIA
Année:
2020
Type:
Article