Evaluation of the Genotoxicity of Endodontic Materials for Deciduous Teeth Using the Comet Assay
Pesqui. bras. odontopediatria clín. integr
; 21: e0060, 2021. tab
Article
de En
| LILACS, BBO
| ID: biblio-1250451
Bibliothèque responsable:
BR1264.1
ABSTRACT
ABSTRACT Objective:
To evaluate genotoxicity of zinc oxide, P. A. calcium hydroxide, mineral trioxide aggregate and an iodoform paste using comet assay on human lymphocytes. Material andMethods:
Two positive controls were used methyl-methanesulfonate for the P.A. calcium hydroxide and mineral trioxide aggregate; and doxorubicin for the iodoform paste and zinc oxide. There were also two negative controls distilled water for the P.A. calcium hydroxide and mineral trioxide aggregate; and DMSO for the iodoform paste and zinc oxide. Comets were identified using fluorescence microscopy and 100 of them were counted on each of the three slides analyzed per drug test. A damage index was established, taking into consideration the score pattern that had previously been determined from the size and intensity of the comet tail. Analysis of variance, followed by Tukey's test, was used to compare the means of the DNA damage indices.Results:
The DNA damage index observed for mineral trioxide aggregate (7.08 to 8.58) and P.A. calcium hydroxide (6.50 to 8.33), which were similar to negative control index. On the other hand, damage index for zinc oxide (104.7 to 218.50) and iodoform paste (115.7 to 210.7) were similar to positive control index.Conclusion:
Iodoform paste and zinc oxide showed genotoxicity at all concentrations used.Mots clés
Texte intégral:
1
Indice:
LILACS
Sujet Principal:
Dent de lait
/
Oxyde de zinc
/
Test des comètes
/
Génotoxicité
/
Tests de mutagénicité
Type d'étude:
Evaluation_studies
/
Prognostic_studies
Limites du sujet:
Humans
Pays comme sujet:
America do sul
/
Brasil
langue:
En
Texte intégral:
Pesqui. bras. odontopediatria clín. integr
Thème du journal:
ODONTOLOGIA
Année:
2021
Type:
Article