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Transplantation of rat-derived microglial cells promotes functional recovery in a rat model of spinal cord injury
Kou, Dewei; Li, Tianmi; Liu, Hong; Liu, Chuansheng; Yin, Yanwei; Wu, Xing; Yu, Tengbo.
Affiliation
  • Kou, Dewei; Hospital of Qingdao University. Department of Pain. Qingdao. CN
  • Li, Tianmi; Hospital of Qingdao University. Operating Room. Qingdao. CN
  • Liu, Hong; Hospital of Qingdao University. Department of Pain. Qingdao. CN
  • Liu, Chuansheng; Hospital of Qingdao University. Department of Pain. Qingdao. CN
  • Yin, Yanwei; Hospital of Qingdao University. Department of Pain. Qingdao. CN
  • Wu, Xing; Hospital of Qingdao University. Department of Pain. Qingdao. CN
  • Yu, Tengbo; Hospital of Qingdao University. Department of Sports Medicine. Qingdao. CN
Rev. bras. pesqui. méd. biol ; Braz. j. med. biol. res;51(10): e7076, 2018. graf
Article de En | LILACS | ID: biblio-951718
Bibliothèque responsable: BR1.1
ABSTRACT
This study evaluated the effect of microglia transplantation on neurological functional recovery in rats subjected to traumatic spinal cord injury (SCI). The rat model of SCI was established using a weight drop device. Forty SCI rats were randomly divided into the microglia group and the saline group. Then, rat-derived microglial cells or normal saline was injected into the injured site 7 days after surgery. The Basso-Beattie-Bresnahan (BBB) score, inclined plate test, and motor-evoked potentials (MEPs) were applied to assess the recovery of motor function. Hematoxylin and eosin (H&E) staining was used to assess the therapeutic effect. Microglia transplantation significantly improved BBB scores and functional scores at 2, 3, 4, 6, and 8 weeks after surgery compared to saline injection (P<0.05). Meanwhile, a prolonged MEP latency and decreased MEP amplitude were observed at 4 and 8 weeks in the microglia group (P<0.05). Histological analysis showed less damage and better prognosis in SCI rats of the microglia group. BrdU+ cell tracing experiments showed that microglia were recruited to the injured area of the spinal cord at 7 and 14 days after transplantation. The intensity of immunofluorescence was increased in CD68+ and OX42+ microglia at 2 days, 1 week, and 2 weeks, and then decreased at 3 and 4 weeks after transplantation in the microglia group. The transplantation of activated microglia played a key role in promoting the recovery of spinal cord function in a rat model of SCI.
Sujet(s)
Mots clés

Texte intégral: 1 Indice: LILACS Sujet Principal: Traumatismes de la moelle épinière / Microglie / Récupération fonctionnelle Type d'étude: Prognostic_studies Limites du sujet: Animals langue: En Texte intégral: Braz. j. med. biol. res / Rev. bras. pesqui. méd. biol Thème du journal: BIOLOGIA / MEDICINA Année: 2018 Type: Article

Texte intégral: 1 Indice: LILACS Sujet Principal: Traumatismes de la moelle épinière / Microglie / Récupération fonctionnelle Type d'étude: Prognostic_studies Limites du sujet: Animals langue: En Texte intégral: Braz. j. med. biol. res / Rev. bras. pesqui. méd. biol Thème du journal: BIOLOGIA / MEDICINA Année: 2018 Type: Article