Mycobacterium bovis: polymerase chain reaction identification in bovine Lymphonode biopsies and genotyping in isolates from Southeast Brazil by spolygotyping and restriction fragment length polymorphism
Mem. Inst. Oswaldo Cruz
; 96(6): 809-813, Aug. 2001. ilus, graf
Article
de En
| LILACS
| ID: lil-298602
Bibliothèque responsable:
BR1.1
ABSTRACT
Diagnosis of the Mycobacterium tuberculosis complex by direct PCR of mediastinal lymphnode DNA and microbiological tests were compared in cattle suspicious of bearing tuberculous-like lesions detected during slaughter. The PCR procedure applied on DNA samples (n=54) obtained by adding alpha -casein into the thiocyanate extraction mix was positive in 70 percent of the samples. PCR confirmed the identification of 23 samples (100 percent) that grew in culture, 9 samples (60 percent) that failed to grow in culture, plus 6 (37.5 percent) samples that resulted in growth of bacterial contaminants. Genotyping by IS6110-RFLP and DR-spoligotyping analysis of seven samples revealed the presence of several polimorphisms. Seven of the isolates contained multiple copies of IS6110, thus defining the existence of five singular genotypes
Texte intégral:
1
Indice:
LILACS
Sujet Principal:
Noeuds lymphatiques
/
Mycobacterium bovis
Type d'étude:
Diagnostic_studies
Limites du sujet:
Animals
Pays comme sujet:
America do sul
/
Brasil
langue:
En
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
MEDICINA TROPICAL
/
PARASITOLOGIA
Année:
2001
Type:
Article