A comparison of silver staining protocols for detecting DNA in polyester-backed polyacrylamide gel
Braz. j. microbiol
; 43(2): 649-652, Apr.-June 2012. ilus, tab
Article
Dans En
| LILACS
| ID: lil-644482
Responsable en Bibliothèque :
BR32.1
ABSTRACT
Eight silver-staining protocols were applied to detect DNA in polyester-backed gels to select the optimal. Results showed important differences in staining quality and that four methods were well-suited for TGGE gels due to high sensitivity and low background, including the Bassam et al. methods, the manufacturer method and our improved method.
Texte intégral:
1
Indice:
LILACS
Sujet Principal:
Polyesters
/
Argent
/
Coloration et marquage
/
Techniques in vitro
/
ADN
/
Alcalanité du Sol
/
Agents colorants
/
Gels
Type d'étude:
Guideline
langue:
En
Texte intégral:
Braz. j. microbiol
Thème du journal:
MICROBIOLOGIA
Année:
2012
Type:
Article
/
Project document