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Detection of immunogenic proteins from Anopheles sundaicussalivary glands in the human serum
Armiyanti, Yunita; Nuryady, Mohammad Mirza; Arifianto, Renam Putra; Nurmariana, Elisa; Senjarini, Kartika; Fitri, Loeki Enggar; Sardjono, Teguh Wahju.
Affiliation
  • Armiyanti, Yunita; Jember University. Faculty of Medicine. Department of Parasitology. Jember. ID
  • Nuryady, Mohammad Mirza; Jember University. Faculty of Medicine. Department of Parasitology. Jember. ID
  • Arifianto, Renam Putra; Jember University. Faculty of Medicine. Department of Parasitology. Jember. ID
  • Nurmariana, Elisa; Jember University. Faculty of Medicine. Department of Parasitology. Jember. ID
  • Senjarini, Kartika; Jember University. Faculty of Medicine. Department of Parasitology. Jember. ID
  • Fitri, Loeki Enggar; Jember University. Faculty of Medicine. Department of Parasitology. Jember. ID
  • Sardjono, Teguh Wahju; Jember University. Faculty of Medicine. Department of Parasitology. Jember. ID
Rev. Soc. Bras. Med. Trop ; Rev. Soc. Bras. Med. Trop;48(4): 410-416, July-Aug. 2015. ilus
Article de En | LILACS | ID: lil-755963
Bibliothèque responsable: BR1.1
ABSTRACT
Abstract<h2>INTRODUCTION:</h2>

The saliva of mosquitoes has an important role in the transmission of several diseases, including malaria, and contains substances with vasomodulating and immunomodulating effects to counteract the host physiological mechanisms and enhance pathogen transmission. As immunomodulatory components, salivary gland proteins can induce the generation of specific IgG antibodies in the host, which can be used as specific biomarkers of exposure to Anopheles sundaicus . The objective of this study was to identify immunogenic proteins from the salivary glands of Anopheles sundaicus by reaction with sera from individuals living in malaria-endemic areas who are thus exposed to Anopheles mosquitoes.

<h2>METHODS:</h2>

IgG antibodies targeting salivary gland proteins in serum samples from individuals living in malaria-endemic areas were measured by enzyme-linked immunosorbent assay (ELISA). Sera from healthy individuals living in non-endemic areas were used as negative controls. Determination of the presence of salivary gland immunogenic proteins was carried out by western blotting.

<h2>RESULTS:</h2>

Sixteen bands appeared in sodium dodecyl sulfate polyacrylamide gel electrophoresis, with molecule weights ranging from 22 to 144kDa. Among the exposed individuals, IgG responses to salivary gland proteins were variable. Protein bands with molecular weights of 46, 41, 33, and 31kDa were the most immunogenic. These immunogenic proteins were consistently recognized by pooled serum and individual samples from people living in malaria-endemic areas but not by negative controls.

<h2>CONCLUSIONS:</h2>

These results support the potential use of immunogenic proteins from the salivary glands of Anopheles as candidate markers of bite exposure or in malaria vaccines.

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Sujet(s)
Mots clés

Texte intégral: 1 Indice: LILACS Sujet Principal: Glandes salivaires / Protéines d'insecte / Anopheles Type d'étude: Diagnostic_studies / Observational_studies / Prognostic_studies Limites du sujet: Animals / Female / Humans langue: En Texte intégral: Rev. Soc. Bras. Med. Trop Thème du journal: MEDICINA TROPICAL Année: 2015 Type: Article

Texte intégral: 1 Indice: LILACS Sujet Principal: Glandes salivaires / Protéines d'insecte / Anopheles Type d'étude: Diagnostic_studies / Observational_studies / Prognostic_studies Limites du sujet: Animals / Female / Humans langue: En Texte intégral: Rev. Soc. Bras. Med. Trop Thème du journal: MEDICINA TROPICAL Année: 2015 Type: Article