Whole-genome sequencing of a Plasmodium vivax isolate from the China-Myanmar border area
Mem. Inst. Oswaldo Cruz
; 110(6): 814-816, Sept. 2015. tab, graf
Article
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| LILACS
| ID: lil-763088
Responsable en Bibliothèque :
BR1.1
ABSTRACT
Currently, there is a trend of an increasing number of Plasmodium vivaxmalaria cases in China that are imported across its Southeast Asia border, especially in the China-Myanmar border area (CMB). To date, little is known about the genetic diversity of P. vivaxin this region. In this paper, we report the first genome sequencing of a P. vivaxisolate (CMB-1) from a vivax malaria patient in CMB. The sequencing data were aligned onto 96.43% of the P. vivaxSalvador I reference strain (Sal I) genome with 7.84-fold coverage as well as onto 98.32% of 14 Sal I chromosomes. Using the de novoassembly approach, we generated 8,541 scaffolds and assembled a total of 27.1 Mb of sequence into CMB-1 scaffolds. Furthermore, we identified all 295 known virgenes, which is the largest subtelomeric multigene family in malaria parasites. These results provide an important foundation for further research onP. vivaxpopulation genetics.
Texte intégral:
1
Indice:
LILACS
Sujet Principal:
Plasmodium vivax
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ADN des protozoaires
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Analyse de séquence d'ADN
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Génome de protozoaire
Pays comme sujet:
Asia
langue:
En
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
MEDICINA TROPICAL
/
PARASITOLOGIA
Année:
2015
Type:
Article
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Project document