Expression of cry1Ab gene from a novel Bacillus thuringiensis strain SY49-1 active on pest insects
Braz. j. microbiol
; Braz. j. microbiol;47(3): 597-602, July-Sept. 2016. graf
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| ID: lil-788966
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ABSTRACT
ABSTRACT In this study, the cry1Ab gene of previously characterized and Lepidoptera-, Diptera-, and Coleoptera-active Bacillus thuringiensis SY49-1 strain was cloned, expressed and individually tested on Ephestia kuehniella (Lepidoptera Pyralidae) and Plodia interpunctella (Lepidoptera Pyralidae) larvae. pET-cry1Ab plasmids were constructed by ligating the cry1Ab into pET28a (+) expression vector. Constructed plasmids were transferred to an Escherichia coli BL21 (DE3) strain rendered competent with CaCl2. Isopropyl β-D-1-thiogalactopyranoside was used to induce the expression of cry1Ab in E. coli BL21(DE3), and consequently, ∼130 kDa of Cry1Ab was obtained. Bioassay results indicated that recombinant Cry1Ab at a dose of 1000 µg g-1 caused 40% and 64% mortality on P. interpunctella and E. kuehniella larvae, respectively. However, the mortality rates of Bt SY49-1 strains' spore-crystal mixture at the same dose were observed to be 70% on P. interpunctella and 90% on E. kuehniella larvae. The results indicated that cry1Ab may be considered as a good candidate in transgenic crop production and as an alternative biocontrol agent in controlling stored product moths.
Mots clés
Texte intégral:
1
Indice:
LILACS
Sujet Principal:
Bacillus thuringiensis
/
Protéines bactériennes
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Expression des gènes
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Lutte contre les insectes
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Endotoxines
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Hémolysines
Limites du sujet:
Animals
langue:
En
Texte intégral:
Braz. j. microbiol
Thème du journal:
MICROBIOLOGIA
Année:
2016
Type:
Article