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Comparative study on determination of alpha-hydroxybutyrate dehydrogenase, lactate dehydrogenase isoenzyme LDH-1 by adsorption on DEAE Sephadex A-50 & electrophoresis.
Article de En | IMSEAR | ID: sea-22856
Serum alpha-hydroxybutyrate dehydrogenase (HBDH) and lactate dehydrogenase isoenzyme (LDH-1) levels by two techniques viz., adsorption on DEAE Sephadex A-50 and by electrophoresis were estimated in healthy subjects and patients with testicular tumours. The correlation coefficient between HBDH and LDH-1 by adsorption technique was found to be r = 0.8. HBDH was found to have a value almost twice that of LDH-1 by adsorption and electrophoretic technique and the activity of LDH-1 obtained by two methods and of HBDH in normals and patients with testicular tumours could be converted using a factor of 0.557. The quantitative method used for determination of HBDH is reliable, accurate, simple and rapid and therefore has better value in a clinical setting than electrophoresis and adsorption techniques which are laborious and time consuming.
Sujet(s)
Texte intégral: 1 Indice: IMSEAR Sujet Principal: Tumeurs du testicule / Humains / Mâle / Chromatographie d'échange d'ions / Électrophorèse / Hydroxybutyrate dehydrogenase / Isoenzymes / L-Lactate dehydrogenase langue: En Année: 1992 Type: Article
Texte intégral: 1 Indice: IMSEAR Sujet Principal: Tumeurs du testicule / Humains / Mâle / Chromatographie d'échange d'ions / Électrophorèse / Hydroxybutyrate dehydrogenase / Isoenzymes / L-Lactate dehydrogenase langue: En Année: 1992 Type: Article