Comparative horizontal starch gel isoenzyme electrophoresis of Lymnaea (Bullastra) cumingiana (Pulmonata: Lymnaeidae) and related taxa in the Indo-Pacific region.
Southeast Asian J Trop Med Public Health
; 1994 Mar; 25(1): 181-6
Article
de En
| IMSEAR
| ID: sea-30920
Foot muscle tissue extracts from six lymnaeid species of the Indo-Pacific region [Lymnaea (Bullastra) cumingiana and L. (Radix) quadrasi from the Philippines, L. (R.) rubiginosa from Indonesia and Thailand, and L. (R.) viridis from Guam and Hong Kong] were subjected to horizontal starch gel isoenzyme electrophoresis and assayed for seven isoenzymes (AcP, AlP, CA, EST, LAP, CAT and GOT) to elucidate their taxonomic relationships. L. cumingiana exhibited banding patterns for EST, LAP and CAT uniquely different from the rest, thus supporting the hypothesis that it is a distinct species. Zymogram patterns for AlP, CA, EST and LAP attest to the close affinity between L. quadrasi and L. rubiginosa (Indonesia and Thailand). Minor differences suggest a closer relationship between the two geographical strains of L. rubiginosa than with L. quadrasi, lending support to the hypothesis that L. quadrasi is inseparable as a race or variety from the typical L. swinhoei Adams, which in turn is but a race of L. auricularia, which also encompasses L. rubiginosa. The two geographical strains of L. viridis from Guam and Hong Kong showed the greatest consistency with regards to similarity and congruence in banding patterns. Non-specific esterases (EST) were the most useful in distinguishing the six species from each other.
Texte intégral:
1
Indice:
IMSEAR
Sujet Principal:
Asie du Sud-Est
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Spécificité d'espèce
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Électrophorèse sur gel d'amidon
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Guam
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Hong Kong
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Isoenzymes
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Lymnea
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Animaux
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Muscles
Type d'étude:
Incidence_studies
Pays comme sujet:
Asia
langue:
En
Texte intégral:
Southeast Asian J Trop Med Public Health
Année:
1994
Type:
Article