In vitro effect of artesunate against Acanthamoeba spp.
Southeast Asian J Trop Med Public Health
; 2002 ; 33 Suppl 3(): 49-52
Article
de En
| IMSEAR
| ID: sea-30927
The in vitro effects of artesunate, the antimalarial agent, and metronidazole against Acanthamoeba spp were studied. Acanthamoeba Group II and Acanthamoeba polyphaga-like were isolated from natural water courses in Buri Ram Province, northeastern Thailand. The trophozoites were axenically cultured in PPYG medium and treated with artesunate in a concentration of 5-700 microg/ml. Artesunate showed its ability to inhibit the growth of acanthamoeba trophozoites: 54% at 50 mg/ml (after six days of exposure) and 93.2% at 100 microg/ml (after two days). The 500-700 microg/ml concentration caused inhibition on the first day of more than 93.2%; excystation did not occur in drug-treated medium. The present study shows that artesunate is amebastatic rather than amebicidal in an axenic culture of trophozoites at the highest concentration of 100 microg/ml. Metronidazole, in concentrations of 5-1,000 microg/ml, had no effects on either trophozoites or cysts.
Texte intégral:
1
Indice:
IMSEAR
Sujet Principal:
Sesquiterpènes
/
Acanthamoeba
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Artémisinines
/
Antiamibiens
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Animaux
langue:
En
Texte intégral:
Southeast Asian J Trop Med Public Health
Année:
2002
Type:
Article