Retroviral reverse transcriptase inhibitory activity in Thai herbs and spices: screening with Moloney murine leukemia viral enzyme.
Southeast Asian J Trop Med Public Health
; 1993 Dec; 24(4): 751-5
Article
de En
| IMSEAR
| ID: sea-33948
Fifty-seven Thai herbs and spices were examined for their retroviral reverse transcriptase inhibitory activity. All herbs and spices were extracted with hot-water and methanol. Reverse transcriptase inhibitory activity of the extracts was determined by using Moloney Murine Leukemia Virus reverse transcriptase (M-MuLV-RT) reacted with 3H-dTTP and radioactivity measured with a scintillation counter. Eighty-one per cent (46/57) of hot-water extracts and 54% (31/57) of methanol extracts showed inhibitory activities. At a concentration of 125 micrograms/ml, 13% (6/46) of hot-water extracts, namely Eugenia caryophyllus Bullock et Harrison, Phyllanthus urinaria Linn., Terminalia belerica Roxb., Nelumbo nucifera Gaertn., Psidium guajava Linn. and Lawsonia inermis Linn., had a relative inhibitory ratio (IR) over 50%. They showed ratios of 100%, 91%, 75%, 74%, 61% and 60%, respectively. For methanol extracts, only 10% (3/31) had IR values over 50%. They were T. belerica, E. caryophyllus and N. nucifera which exhibited IR values of 83%, 54% and 54%, respectively.
Texte intégral:
1
Indice:
IMSEAR
Sujet Principal:
Plantes médicinales
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Retroviridae
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Thaïlande
/
Épices
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Virus de la leucémie murine
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Inhibiteurs de la transcriptase inverse
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Antienzymes
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Animaux
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Souris
Type d'étude:
Diagnostic_studies
/
Screening_studies
Pays comme sujet:
Asia
langue:
En
Texte intégral:
Southeast Asian J Trop Med Public Health
Année:
1993
Type:
Article