Antibiotic resistance, plasmid profile and RAPD-PCR analysis of enteropathogenic Escherichia coli (EPEC) clinical isolates.
Southeast Asian J Trop Med Public Health
; 2003 Sep; 34(3): 620-6
Article
Dans En
| IMSEAR
| ID: sea-35942
ABSTRACT
Enteropathogenic Escherichia coli (EPEC) is a leading cause of diarrhea among infants in developing countries. A total of 38 EPEC isolates, obtained from diarrhea patients of Hospital Miri, Sarawak, were investigated through plasmid profile, antibiotic resistance and randomly amplified polymorphic DNA (RAPD) analysis. From the 8 types of antibiotics used, all isolates were 100% resistant to furoxime, cephalothin and sulphamethoxazole and showed high multiple antibiotic resistant (MAR) indexes, ranging from 0.5 to 1.0. In plasmid profiling, 22 isolates (58%) showed the presence of one or more plasmids in the range 1.0 to 30.9 mDa. The dendrogram obtained from the results of the RAPD-PCR discriminated the isolates into 30 single isolates and 3 clusters at the level of 40% similarity. The EPEC isolates were highly diverse, as shown by their differing plasmid profiles, antibiotic resistance patterns and RAPD profiles.
Texte intégral:
1
Indice:
IMSEAR
Sujet Principal:
Plasmides
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Humains
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Résistance aux substances
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Tests de sensibilité microbienne
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Valeur prédictive des tests
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Profilage d'ADN
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Technique RAPD
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Adulte
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Diarrhée
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Escherichia coli
Type d'étude:
Evaluation_studies
Pays comme sujet:
Asia
langue:
En
Texte intégral:
Southeast Asian J Trop Med Public Health
Année:
2003
Type:
Article