Quality comparison of cold cryoprecipitates in two different volumes / 中国输血杂志

ABSTRACT

【Objective】 To study the concentration, content and yield of coagulation factor Ⅷ(FⅧ) and fibrinogen(Fg) in 25 mL and 45 mL cryoprecipitate. 【Methods】 Forty aliquots(200 mL fresh whole blood) were divided into group A and group B, with 20 samples in each group. Fresh frozen plasma(FFP) was prepared within 6 hours(anticoagulant ACD) according to the standard operating procedure for component preparation. After one week, the prepared FFP was prepared into(25±5) mL and(45±5) mL cold precipitates by siphon method. After freezing for one week, the concentrations of FⅧ and Fg were detected after meltingin by water bath at 37℃, and the content and yield were calculated. 【Results】 The FⅧ concentration, content and yield in group A and group B were(2.990±0.988) vs(2.744±0.940) IU/mL, (74.75±24.71) vs(113.75±30.06)IU, and(70.1±16.6) vs(85.0±7.6)%, respectively.The Fg concentration, content and yield was(6.013±1.679) vs(5.844±0.683) g/L, (150.33±41.99) vs(252.23±26.90)mg, and(41.7±8.6) vs (49.1±9.6)%, respectively. Statistical analysis suggested that the content and yield of FⅧ and Fg were statistically different between the two groups(P0.05). 【Conclusion】 FⅧ and Fg of low-volume cryoprecipitate presented lower content and yield, but slightly higher concentration. Both products can meet the quality requirements of whole blood and blood component. Therefore, reducing product capacity appropriately is suggested when preparing cryoprecipitate with 200mL whole blood