Efficient synthesis of L-methionine by engineering the one carbon module of Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology
; (12): 3302-3317, 2023.
Article
Dans Zh
| WPRIM
| ID: wpr-1007959
Responsable en Bibliothèque :
WPRO
ABSTRACT
L-methionine, also known as L-aminomethane, is one of the eight essential amino acids required by the human body and has important applications in the fields of feed, medicine, and food. In this study, an L-methionine high-yielding strain was constructed using a modular metabolic engineering strategy based on the M2 strain (Escherichia coli W3110 ΔIJAHFEBC/PAM) previously constructed in our laboratory. Firstly, the production of one-carbon module methyl donors was enhanced by overexpression of methylenetetrahydrofolate reductase (methylenetetrahydrofolate reductase, MetF) and screening of hydroxymethyltransferase (GlyA) from different sources, optimizing the one-carbon module. Subsequently, cysteamine lyase (hydroxymethyltransferase, MalY) and cysteine internal transporter gene (fliY) were overexpressed to improve the supply of L-homocysteine and L-cysteine, two precursors of the one-carbon module. The production of L-methionine in shake flask fermentation was increased from 2.8 g/L to 4.05 g/L, and up to 18.26 g/L in a 5 L fermenter. The results indicate that the one carbon module has a significant impact on the biosynthesis of L-methionine, and efficient biosynthesis of L-methionine can be achieved through optimizing the one carbon module. This study may facilitate further improvement of microbial fermentation production of L-methionine.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Carbone
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Protéines de transport
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Hydroxymethyl et formyl transferases
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Cystéine
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Protéines Escherichia coli
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Methylenetetrahydrofolate reductase (NADPH2)
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Escherichia coli
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Méthionine
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Chinese Journal of Biotechnology
Année:
2023
Type:
Article