Dual flow immunochromatographic assay for rapid and simultaneous quantitative detection of ochratoxin A and zearalenone in corn, wheat, and feed samples / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
J. Zhejiang Univ., Sci. B (Internet)
; (12): 871-883, 2018.
Article
de En
| WPRIM
| ID: wpr-1010428
Bibliothèque responsable:
WPRO
ABSTRACT
A one-step dual flow immunochromatographic assay (DICGA), based on a competitive format, was developed for simultaneous quantification of ochratoxin A (OTA) and zearalenone (ZEN) in corn, wheat, and feed samples. The limit of detection for OTA was 0.32 ng/ml with a detection range of 0.53‒12.16 ng/ml, while for ZEN it was 0.58 ng/ml with a detection range of 1.06‒39.72 ng/ml. The recovery rates in corn, wheat, and feed samples ranged from 77.3% to 106.3% with the coefficient of variation lower than 15%. Naturally contaminated corn, wheat, and feed samples were analyzed using both DICGA and liquid chromatography-tandem mass spectrometry (LC-MS/MS) and the correlation between the two methods was evaluated using a regression analysis. The DICGA method shows great potential for simple, rapid, sensitive, and cost-effective quantitative detection of OTA and ZEN in food safety control.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Triticum
/
Zéaralénone
/
Calibrage
/
Dosage immunologique
/
Contamination des aliments
/
Analyse de régression
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Reproductibilité des résultats
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Chromatographie d'affinité
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Chromatographie en phase liquide
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Sensibilité et spécificité
langue:
En
Texte intégral:
J. Zhejiang Univ., Sci. B (Internet)
Année:
2018
Type:
Article