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Dual flow immunochromatographic assay for rapid and simultaneous quantitative detection of ochratoxin A and zearalenone in corn, wheat, and feed samples / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Article de En | WPRIM | ID: wpr-1010428
Bibliothèque responsable: WPRO
ABSTRACT
A one-step dual flow immunochromatographic assay (DICGA), based on a competitive format, was developed for simultaneous quantification of ochratoxin A (OTA) and zearalenone (ZEN) in corn, wheat, and feed samples. The limit of detection for OTA was 0.32 ng/ml with a detection range of 0.53‒12.16 ng/ml, while for ZEN it was 0.58 ng/ml with a detection range of 1.06‒39.72 ng/ml. The recovery rates in corn, wheat, and feed samples ranged from 77.3% to 106.3% with the coefficient of variation lower than 15%. Naturally contaminated corn, wheat, and feed samples were analyzed using both DICGA and liquid chromatography-tandem mass spectrometry (LC-MS/MS) and the correlation between the two methods was evaluated using a regression analysis. The DICGA method shows great potential for simple, rapid, sensitive, and cost-effective quantitative detection of OTA and ZEN in food safety control.
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Mots clés
Texte intégral: 1 Indice: WPRIM Sujet Principal: Triticum / Zéaralénone / Calibrage / Dosage immunologique / Contamination des aliments / Analyse de régression / Reproductibilité des résultats / Chromatographie d'affinité / Chromatographie en phase liquide / Sensibilité et spécificité langue: En Texte intégral: J. Zhejiang Univ., Sci. B (Internet) Année: 2018 Type: Article
Texte intégral: 1 Indice: WPRIM Sujet Principal: Triticum / Zéaralénone / Calibrage / Dosage immunologique / Contamination des aliments / Analyse de régression / Reproductibilité des résultats / Chromatographie d'affinité / Chromatographie en phase liquide / Sensibilité et spécificité langue: En Texte intégral: J. Zhejiang Univ., Sci. B (Internet) Année: 2018 Type: Article