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Rapamycin attenuates ioversol-induced acute kidney injury in rat models / 基础医学与临床
Basic & Clinical Medicine ; (12): 31-36, 2024.
Article Dans Zh | WPRIM | ID: wpr-1018568
Responsable en Bibliothèque : WPRO
ABSTRACT
Objective To investigate the role of autophagy in contrast-induced acute kidney injury(CI-AKI)in rats and to explore its possible mechanism.Methods SD rats were randomly divided into control group,acute kid-ney injury model group(intravenous injection of contrast medium ioversol via tail vein;model),rapamycin(RAPA)group and hydroxychloroquine(HCQ)group.Blood urea nitrogen(BUN)and serum creatinine(Scr)con-tents were measured and the potential change foun in renal pathology was detected by HE staining and microscopy.Transmission electron microscopy was used to observe auto-phagy-related changes in ultrastructure.Western blot was used to observe the expression of microtubule-associated protein 1 light chain 3(LC3)Ⅱ/LC3Ⅰ,ubiquitin-binding protein p62 and Histone deacetylase 4(HDAC4).The expression of HDAC4 was also observed by RT-qPCR.Results Compared with control group,the level of BUN,Scr and HDAC4 expression in the model and HCQ group was increased(P<0.01),the proximal tubules of the kidney were significantly damaged.In the model group,auto-phagososomes and autolysosomes increased,accompanied by an increase of LC3Ⅱ/LC3Ⅰ and a decrease in the p62 level(P<0.05,P<0.01);Compared with model group,there were more autophagosomes and autolysosomes were found in RAPA group(P<0.01),accompanied by increased LC3Ⅱ/LC3Ⅰratio and decrease in the p62 and HDAC4(P<0.05,P<0.01).In contrast,the number of autophagy related structures decreased in HCQ group(P<0.01),accompanied by the simultaneous increase of LC3Ⅱ/LC3Ⅰ,p62 and the increase of HDAC4(P<0.01).Conclusions Ioversol may induce autophagy activation,while enhancing autophagy by RAPA alleviates CI-AKI induced renal dysfunction.The mechanism is potentially atributed to the regulation of HDAC4.

Texte intégral: 1 Indice: WPRIM langue: Zh Texte intégral: Basic & Clinical Medicine Année: 2024 Type: Article
Texte intégral: 1 Indice: WPRIM langue: Zh Texte intégral: Basic & Clinical Medicine Année: 2024 Type: Article