The influence of static mechanical strain on osteoclastogenic gene expression of HPDLSCs and PPDLSCs / 实用口腔医学杂志
Journal of Practical Stomatology
; (6): 257-262, 2024.
Article
de Zh
| WPRIM
| ID: wpr-1020571
Bibliothèque responsable:
WPRO
ABSTRACT
Objective:To explore the influence of static mechanical strain(SMS)on the osteoclastogenic gene expression of healthy periodontal ligment stem cells(HPDLSCs)and periodontitis periodontal ligment stem cells(PPDLSCs).Methods:HPDLSCs and PP-DLSCs were respectively isolated and cultured by low density in vitro.The expression of mesenchymal stem cell markers were detected by flow cytometry.Then,6%,8%,10%,12%and 14%SMS were respectively loaded to the HPDLSCs and PPDLSCs by Flexcell Tension Unit,and the expression of RANKL and C-fos was detected by real time RT-PCR.Results:Both HPDLSCs and PPDLSCs strongly expressed the mesenchymal stem cell markers STRO-1,CD146,CD90 and CD29,and higher expression of the above markers was found in HPDLSCs compared with PPDLSCs(P<0.05).The expression of RANKL and C-fos in PPDLSCs was more obvious than that in HPDLSCs without SMS loading(P<0.05).For HPDLSCs,the SMS of 14%induced significant up-regulation of RANKL and C-fos(P<0.05),while no alteration was confirmed for the above osteoclastogenic genes when the SMS≤ 12%(P>O.05).In addition,the expression of RANKL and C-fos was up-regulated significantly in PPDLSCs when the SMS≥ 10%(P<0.05),and the expression of the above genes was not activated when the SMS ≤8%.Conclusion:HPDLSCs and PPDLSCs response differently to SMS,and ex-cessive SMS may lead to enhanced expression of osteoclastogenic genes in PPDLSCs.
Texte intégral:
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Indice:
WPRIM
langue:
Zh
Texte intégral:
Journal of Practical Stomatology
Année:
2024
Type:
Article