Protective effect of fructooligosaccharides on intestinal mucosal barrier in mice with ulcerative colitis / 中国小儿急救医学

ABSTRACT

Objective:To investigate the protective effect of water-soluble dietary fiber fructooligosaccharides(FOS) on intestinal mucosal barrier in mice with ulcerative colitis(UC), and to find a new drug option for the treatment of patients with UC.Methods:This study used 4% dextran sodium sulfate(DSS)to induce a 7-day UC mouse model.Male C57BL/6 mice aged 6-8 weeks were randomly divided into three groups: the control group drank distilled water; The model group was given 4% DSS; The oligofructose group was administered 20 mg/mL FOS by gavage simultaneously with DSS induction.The body weight, fecal characteristics, and fecal blood status of mice daily, and the disease activity index(DAI)score were monitored.After the experiment, HE staining was used to observe the pathological changes in the colon tissue of mice.Real time fluorescence quantitative PCR, Western Blot, and immunofluorescence staining were used to detect the expression levels of tight junction proteins ZO-1, Claudin-1, and Occludin in the intestines of each group of mice.Results:Compared with control group, the weight of mice decreased and the DAI score increased in model group( P<0.05). However, the weight of mice in FOS group was higher than that in model group, and the DAI score was lower than that in model group ( P<0.05). Compared with control group, the colon length of mice in model group was shortened[(7.52±0.41)cm vs.(5.48±0.19)cm], and the histopathological score was increased (0.53±0.38 vs.3.51±0.18). However, the colon length of mice in FOS group[(6.82±0.63)cm] was longer than that in model group, and the hispathological score(2.33±0.63) was lower than that in model group.All the differences were statistically significant ( P<0.05). The expression levels of ZO-1, Claudin-1, Occludin protein and its mRNA levels in model group were lower than those in control group ( P<0.05). However, those of FOS group were higher than those in model group ( P<0.05). Conclusion:FOS could alleviate the symptoms of weight loss in UC mice, reduce their DAI score, improve inflammation of colon tissue, upregulate the expression of tight junction proteins ZO-1, Claudin-1, and Occludin, and protect the intestinal mucosal barrier.