Screening of key genes and pathway and analytical research of traditional Chinese medicines for primary immune thrombocytopenia based on bioinformatics analysis / 中国免疫学杂志

ABSTRACT

Objective:To preliminarily screen the key genes of primary immune thrombocytopenia(ITP)by bioinformatics method and explore the pathogenesis,so as to predict the potential traditional Chinese medicine(TCM)for the treatment of ITP.Methods:Based on the original microarray data set GSE80401 under the National Center for Biotechnology Information(NCBI),the differential miRNA of ITP were obtained by analyzing the adjusted P<0.05 and |logFC|≥1 as the screening criteria for differential miRNA.miRTarBase,miRDB and TargetScan were used to predict miRNA target genes.The target of ITP was searched in Genecards database,and the predicted up-regulated target genes and down-regulated target genes were intersected with disease targets.On this basis,the mapped target genes were respectively constructed into PPI network through String database and Cytoscape to screen core target genes,and the core target genes were enriched and analyzed in DAVID and Omicsbean databases for GO and KEGG pathways.The key genes were imported into the Coremine Medical database to analyze the TCM for the treatment of key genes.Results:Total of 422 differential genes and 17 key genes were finally screened,including BCL2L1,CCND1,CD44,CDKN1A,CREB1,GRB2,MAPK1,MAPK8,PIK3R1,CDK2,CAV1,FGF2,IGF1,SMAD2,SMAD4,TLR4 and VEGFA,mainly involving proteoglycan,FoXO,PI3K-Akt,human T cell leukemia virus 1 infection,endocrine resistance,focal adhesion and other signal pathways.A total of 12 TCM for ITP prevention and treatment,including ginseng,Radix Paeoniae Rubra,Angelica sinensis,bee venom,cobra,Psoralea corylifolia,Rehmannia glutinosa,buffalo horn,hemp seed,dodder seed,Wulingzhi and Jinji NaPi were screened.TLR4 maps the most TCM,followed by CCND1 and VEGFA.Among many TCM,ginseng acts on 17 targets at the same time,Radix Paeoniae Rubra,Angelica sinensis and bee venom act on 11 targets at the same time,cobra and Psoralea corylifolia act on 9 and 8 targets at the same time,Rehmannia glutinosa and buffalo horn act on 7 targets at the same time,hemp seed act on 4 targets at the same time,dodder seed act on 3 targets at the same time,and wulingzhi act on 2 targets at the same time.It is suggested that these drugs had the potential of multi-target prevention and treatment of ITP.Conclusion:The key pathogenic genes of ITP and the TCM with preventive and thera-peutic effects could be preliminarily predicted based on the analysis of genetic information,which can provide targets and research ideas for the development of related TCM.