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Expression of 3b-Hydroxysteroid dehydrogenase and P450 side chain cleavage enzyme in the human uterine endometrium
Exp. mol. med ; Exp. mol. med;: 160-166, 2003.
Article de En | WPRIM | ID: wpr-10316
Bibliothèque responsable: WPRO
ABSTRACT
The enzyme complex 3b-hydroxysteroid dehydrogenase/delta(5)-delta(4)-isomerase (3beta-HSD) is involved in the biosynthesis of all classes of active steroids. The expression of 3beta-HSD in human uterine endometrium during the menstrual cycle and decidua was examined in an effort to understand its role during ova implantation. 3beta-HSD was weakly expressed in the glandular epithelium of the proliferative phase and moderately expressed in the glandular epithelium of secretory phase of the endometrium. In the decidua of the ectopic pregnancy, 3beta-HSD was strongly expressed. The human uterine endometrial 3beta-HSD was identified as being the same type as the placental 3beta-HSD by RT-PCR and sequence analysis. In addition to the expression of 3beta-HSD, P450scc was expressed in the decidua of the ectopic pregnancy. These results suggest that pregnenolone might be synthesized from cholesterol by P450scc de novo and then, it is converted to progesterone by 3beta-HSD in the uterine endometrium. The data implies that the endometrial 3beta-HSD can use not only the out-coming pregnenolone from the adrenal gland but also the self- made pregnenolone to produce progesterone. The de novo synthesis of progesterone in the endometrium might be a crucial factor for implantation and maintenance of pregnancy.
Sujet(s)
Mots clés
Texte intégral: 1 Indice: WPRIM Sujet Principal: Placenta / Prégnénolone / Progestérone / Progesterone reductase / Steroid isomerases / Cholesterol side-chain cleavage enzyme / Expression des gènes / Cholestérol / Caduques / Endomètre Type d'étude: Prognostic_studies Limites du sujet: Female / Humans / Pregnancy langue: En Texte intégral: Exp. mol. med Année: 2003 Type: Article
Texte intégral: 1 Indice: WPRIM Sujet Principal: Placenta / Prégnénolone / Progestérone / Progesterone reductase / Steroid isomerases / Cholesterol side-chain cleavage enzyme / Expression des gènes / Cholestérol / Caduques / Endomètre Type d'étude: Prognostic_studies Limites du sujet: Female / Humans / Pregnancy langue: En Texte intégral: Exp. mol. med Année: 2003 Type: Article