Effect of miR-141-3p targeting HMGB1 on LPS-induced A549 cell injury / 安徽医科大学学报
Acta Universitatis Medicinalis Anhui
; (6): 85-91, 2024.
Article
Dans Zh
| WPRIM
| ID: wpr-1032158
Responsable en Bibliothèque :
WPRO
ABSTRACT
Objective @#To investigate the effect of miR-141-3p on LPS induced A549 cell injury by targeting high mobility group protein 1 (HMGB1) .@*Methods @#A549 cells derived from type Ⅱ alveolar epithelial cells were taken as the study object,miR-141-3p mimics,mimics NC,HMGB1 gene overexpression plasmid (pcDNA3. 1-HMGB1) and empty Vector were transfected into A549 cells respectively or co-transfected,then 10 μg / ml LPS was used for 24 h.Cell proliferation activity was detected by cell counting kit-8 ( CCK-8) .The activity of lactate dehydrogenase ( LDH) in the supernatant of cell culture was detected by colorimetry.The apoptosis level of each group was detec- ted by flow cytometry.The levels of interleukin (IL) -1 β , IL-6 and tumor necrosis factor α (TNF-α) were detected by enzyme-linked immunosorbent assay (Elisa) .Dual luciferase reporter gene assay verified the targeted regulatory relationship between miR-141-3p and HMGB1 . @*Results @#After treatment with LPS ,the proliferative activity of A549 cells and the expression level of miR-141-3p decreased ( P <0. 05 ) ,the apoptosis rate increased ( P < 0. 05) ,the levels of IL-1 β , IL-6,TNF-α and the activity of LDH in supernatant increased (P<0. 05) .Overex- pression of miR-141-3p increased the proliferation activity of A549 cells treated with LPS (P <0. 05 ) ,and de- creased the apoptosis rate and the levels of IL-1 β , IL-6,TNF-α in cells and LDH activity in supernatant (P < 0. 05) .However,overexpression of HMGB1 gene could reverse the ameliorative effect of miR-141-3p on LPS-in- duced A549 cell injury.Dual luciferase reporter gene experiment confirmed that HMGB1 was the downstream target gene of miR-141-3p.@*Conclusion @# miR-141-3p can inhibit LPS-induced apoptosis,reduce the expression level of inflammatory factors,and improve the damage of A549 cells,which may be related to the targeted regulation of HMGB1 expression.
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langue:
Zh
Texte intégral:
Acta Universitatis Medicinalis Anhui
Année:
2024
Type:
Article