Effect of recombinant human erythropoietin on ATPase activity and free radical metabolism in the neuronal mitochondria after brain injury in rats / 中华神经医学杂志

ABSTRACT

Objective To investigate the effect of recombinant human erythropoietin(rhEPO) on the activity of ATPase and superoxide dismutase(SOD) and malondialdehyde(MDA)content in the mitochondria of rat neurons after brain injury. Methods rhEPO was intraperitoneally injected in rats immediately after brain contusion injury induced by impact of a free-falling object.In the control rats,an equivalent volume of saline was injcoted intraperitoneally after the brain injury.At 6,12,24,and 48 hafter rhEPO or saline injection,the mitochondria were isolated from the brain neurons of the rats to determine the activity of ATPasc and SOD and the content of MDA.Results At 12.24 and48 h after rhEPO trealraent.the activity of ATPase and SOD increased and MDA content decreased significantly in the mitochondria of the brain neurons as compared with the measurements in the control group at the corresponding time points(P<0.05). Conclusion rhEPO treatment can ameliorate secondary brain injury in ratsfollowing brain trauma by modulating the mitochondrial function.