Effect of gap junction blocker octanol on mitogen activated protein kinase signal pathway after cerebral ischemia reperfusion in rats / 中华神经医学杂志

ABSTRACT

Objective To investigate the effect of octanol,a gap junction blocker,on mitogen activated protein kinase (MAPK) signal pathway after ischemia reperfusion in rats.Methods Seventy-two male SD rats were randomly divided into sham-operated group,DMSO control group,saline control group and octanol group (n=1 8).The focal cerebral ischemia reperfusion models were established by suture method in the later three groups.Rats were injected 5 mmol/kg octanol solution (5％ octanol soluble in 5％ DMSO solution) into the abdominal cavity of rats in the octanol group 30 min before ischemia reperfusion;rats in the DMSO control group were injected with same amount of 5％ DMSO solution,and those in the sham-operated group and saline control group were injected with same amount of saline.At 24 h after reperfusion,Neurological Function Defect Scale was performed;water content in brain tissues was detected by dry-wet method;cerebral infarction volume percentage was detected by TTC staining;the total protein expressions of extracellular signal-regulated kinase 1/2 (ERK1/2),c-jun amino-terminal kinase (JNK),and p38,and protein expressions ofphosphorylased (p)-ERK1/2,p-JNK,and p-p38 were detected by Western blotting.Results The scores of Neurological Function Defect Scale,water content in brain tissues,and infarction volume percentage of the octanol group (1.583±0.651,78.363％±0.672％,and 24.34％±0.19％) were obviously reduced as compared with those in the DMSO control group (2.344±0.743,80.873％±0.831％,and 32.26％±0.21％) and saline control group (2.351±0.732,80.893％±0.734％,and 32.28％±0.24％),with significant differences (P＜0.05).The protein expressions ofp-ERKl/2,p-JNK,and p-p38 in the octanol group (0.201±0.009,0.211±0.011,and 0.191±0.009) were obviously reduced as compared with those in the DMSO control group (0.389±0.019,0.311±0.022,and 0.309±0.021) and saline control group (0.393±0.021,0.304±0.021,and 0.316±0.025),with significant differences (P＜0.05).Conclusion Octanol can reduce ischemic reperfusion injury,whose mechanism may be related to the regulation of MAPK signal pathway.