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Bufalin induces apoptosis of HCT116 cells by activating endoplasmic reticulum stress pathway / 安徽医科大学学报
Article de Zh | WPRIM | ID: wpr-1038391
Bibliothèque responsable: WPRO
ABSTRACT
Objective @#To study the effect of bufalin on the proliferation and apoptosis of human colorectal cancer cell line HCT116,and to explore the role of endoplasmic reticulum stress ( ERS) in this process.@*Methods @#The effect of bufalin on the proliferation of HCT116 cells was determined by CCK-8 assay.After HCT116 cells were treated with different concentrations of bufalin for 48 hours,cell apoptosis was detected by Annexin V / PI assay, and the expression of apoptosis-related proteins Bax and Bcl-2 was detected by Western blot.At the same time,the expression of ERS-related proteins glucose regulated protein 78 ( GRP78) ,phosphorylated protein kinase R like endoplasmic reticulum kinase ( p-PERK) ,eukaryotic translation initiation factor 2 α ( eIF2 α) ,phosphorylated eukaryotic translation initiation factor 2 α (p-eIF2 α) and C / EBP homologous protein ( CHOP) was detected by Western blot.HCT116 cells were divided into control group,bufalin group and combination group (bufalin + 4-phenylbutyric acid) ,and the expression of apoptosis-related proteins Bax and Bcl-2 was observed by Western blot. @*Results@#CCK-8 assay showed that bufalin could inhibit the proliferation of HCT116 cells.Apoptosis assay showed that bufalin could induce apoptosis of HCT116 cells.The results of Western blot showed that bufalin could up-regulate the expression of pro-apoptotic protein Bax and down-regulate the expression of anti-apoptotic protein Bcl-2.It could also induce ERS and activate PERK / eIF2 α/ CHOP pathway.When bufalin combined with 4-phenylbutyric acid,the apoptosis-promoting effect of bufalin was inhibited.@*Conclusion@#Bufalin can effectively inhibit the prolif- erative activity and induce apoptosis of HCT116,which is achieved to some extent by activating ERS.
Mots clés
Texte intégral: 1 Indice: WPRIM langue: Zh Texte intégral: Acta Universitatis Medicinalis Anhui Année: 2023 Type: Article
Texte intégral: 1 Indice: WPRIM langue: Zh Texte intégral: Acta Universitatis Medicinalis Anhui Année: 2023 Type: Article