The effects mutant DJ-1 and sumoylation of DJ-1 protein on DJ-1 mitochondrial localization / 中风与神经疾病杂志
Journal of Apoplexy and Nervous Diseases
; (12): 773-776, 2021.
Article
Dans Zh
| WPRIM
| ID: wpr-1039391
Responsable en Bibliothèque :
WPRO
ABSTRACT
@#Objective To investigate the effects of DJ-1 A39S mutation in Parkinson’s disease(PD)and SUMO modification of DJ-1 on DJ-1 mitochondrial localization. Methods Five COS-7 cell lines that can stably express wild type,A39S mutant,K130R mutant,A39S K130R double mutant DJ-1 protein and SUMO-1 protein were constructed. The transfection results were detected by Western blot,double enzyme digestion and PCR sequencing. The expression and subcellular localization of DJ-1 protein were observed by Western blotting,confocal microscope and immunofluorescence.Results Immunofluorescence showed that DJ-1 protein was mainly distributed in the cytoplasm and also in the nucleus.Some wild-type and K130R DJ-1 proteins were distributed in mitochondria,most of A39S and A39S-K130R mutant DJ-1 proteins were transferred to mitochondria,and SUMO-1 was mainly distributed in the nucleus and around the nucleus.Immunofluorescence co localization showed that four DJ-1 proteins were colocalize with SUMO-1 protein. Conclusion The pathogenic mutation A39S of DJ-1 transferred DJ-1 protein from cytoplasm to mitochondria;DJ-1 and SUMO-1 have colocalization,and they may interact.mitochondrial localization.Methods Five COS-7 cell lines that can stably express wild type,A39S mutant,K130R mutant,A39S K130R double mutant DJ-1 protein and SUMO-1 protein were constructed. The transfection results were detected by Western blot,double enzyme digestion and PCR sequencing.The expression and subcellular localization of DJ-1 protein were observed by Western blotting,confocal microscope and immunofluorescence.Results Immunofluorescence showed that DJ-1 protein was mainly distributed in the cytoplasm and also in the nucleus.Some wild type and K130R DJ-1 proteins were distributed in mitochondria,most of A39S and A39S-K130R mutant DJ-1 proteins were transferred to mitochondria,and SUMO-1 was mainly distributed in the nucleus and around the nucleus.Immunofluorescence colocalization showed that four DJ-1 proteins were colocalize with SUMO-1 protein.Conclusion The pathogenic mutation A39S of DJ-1 transferred DJ-1 protein from cytoplasm to mitochondria;DJ-1 and SUMO-1 have colocalization,and they may interact.
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Indice:
WPRIM
langue:
Zh
Texte intégral:
Journal of Apoplexy and Nervous Diseases
Année:
2021
Type:
Article