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Granulocyte Colony Stimulating Factor (G-CSF) Attenuates 2,4,6-Trinitrobenzene Sulfonic Acid (TNBS)-induced Colitis in Mice
Immune Network ; : 13-19, 2006.
Article de Ko | WPRIM | ID: wpr-109770
Bibliothèque responsable: WPRO
ABSTRACT
BACKGROUND: Granulocyte colony stimulating factor (G-CSF) is known as a cytokine central to the hematopoiesis of blood cells and to modulate their cellular functions. Besides granulocytes and their precursors, monocytes/macrophages and endothelial cells are direct target cells of G-CSF action. G-CSF influences immune cells in an anti-inflammatory way. METHODS: To evaluate whether G-CSF has a potential for preventing or ameliorating diseases characterized by mucosal inflammation, we used a mouse model with trinitrobenzene sulfonic acid (TNBS)-induced inflammatory colitis. To the mice model G-CSF was administrated daily by intraperitoneal injection. Macroscopic evaluation and immunohistochemical analysis of colonic tissues were performed. RESULTS: Recombinant human G-CSF significantly inhibited LPS-induced TNF-alpha mRNA expression in THP-1 cells. As for in vivo relevance, G-CSF dramatically reduced the weight loss of mice, colonic damage, and mucosal ulceration that characterize TNBS colitis. Moreover, G-CSF suppressed the expression of tumor necrosis factor-alpha, interleukin-1beta, and intercellular adhesion molecule-1 in TNBS colitis. CONCLUSION: Current results demonstrate that G-CSF may be an effective agent for the treatment of diseases characterized by mucosal inflammation.
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Mots clés
Texte intégral: 1 Indice: WPRIM Sujet Principal: Ulcère / Cellules sanguines / ARN messager / Maladies inflammatoires intestinales / Perte de poids / Facteur de stimulation des colonies de granulocytes / Facteurs de stimulation des colonies / Facteur de nécrose tumorale alpha / Colite / Côlon Type d'étude: Prognostic_studies Limites du sujet: Animals / Humans langue: Ko Texte intégral: Immune Network Année: 2006 Type: Article
Texte intégral: 1 Indice: WPRIM Sujet Principal: Ulcère / Cellules sanguines / ARN messager / Maladies inflammatoires intestinales / Perte de poids / Facteur de stimulation des colonies de granulocytes / Facteurs de stimulation des colonies / Facteur de nécrose tumorale alpha / Colite / Côlon Type d'étude: Prognostic_studies Limites du sujet: Animals / Humans langue: Ko Texte intégral: Immune Network Année: 2006 Type: Article