In vivo Tracking of Mesenchymal Stem Cells Labeled with a Novel Chitosan-coated Superparamagnetic Iron Oxide Nanoparticles using 3.0T MRI
Journal of Korean Medical Science
; : 211-219, 2010.
Article
Dans En
| WPRIM
| ID: wpr-109868
Responsable en Bibliothèque :
WPRO
ABSTRACT
This study aimed to characterize and MRI track the mesenchymal stem cells labeled with chitosan-coated superparamagnetic iron oxide (Chitosan-SPIO). Chitosan-SPIO was synthesized from a mixture of FeCl2 and FeCl3. The human bone marrow derived mesenchymal stem cells (hBM-MSC) were labeled with 50 microg Fe/mL chitosan-SPIO and Resovist. The labeling efficiency was assessed by iron content, Prussian blue staining, electron microscopy and in vitro MR imaging. The labeled cells were also analyzed for cytotoxicity, phenotype and differentiation potential. Electron microscopic observations and Prussian blue staining revealed 100% of cells were labeled with iron particles. MR imaging was able to detect the labeled MSC successfully. Chitosan-SPIO did not show any cytotoxicity up to 200 microgram Fe/mL concentration. The labeled stem cells did not exhibit any significant alterations in the surface markers expression or adipo/osteo/chondrogenic differentiation potential when compared to unlabeled control cells. After contralateral injection into rabbit ischemic brain, the iron labeled stem cells were tracked by periodical in vivo MR images. The migration of cells was also confirmed by histological studies. The novel chitosan-SPIO enables to label and track MSC for in vivo MRI without cellular alteration.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Phénotype
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Imagerie par résonance magnétique
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Composés du fer III
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Différenciation cellulaire
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Encéphalopathie ischémique
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Transplantation de cellules souches mésenchymateuses
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Chitosane
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Nanoparticules métalliques
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Complexes de coordination
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Cellules souches mésenchymateuses
Limites du sujet:
Animals
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Humans
langue:
En
Texte intégral:
Journal of Korean Medical Science
Année:
2010
Type:
Article