A novel M2e-multiple antigenic peptide providing heterologous protection in mice
Journal of Veterinary Science
; : 71-78, 2016.
Article
Dans En
| WPRIM
| ID: wpr-110763
Responsable en Bibliothèque :
WPRO
ABSTRACT
Swine influenza viruses (SwIVs) cause considerable morbidity and mortality in domestic pigs, resulting in a significant economic burden. Moreover, pigs have been considered to be a possible mixing vessel in which novel strains loom. Here, we developed and evaluated a novel M2e-multiple antigenic peptide (M2e-MAP) as a supplemental antigen for inactivated H3N2 vaccine to provide cross-protection against two main subtypes of SwIVs, H1N1 and H3N2. The novel tetra-branched MAP was constructed by fusing four copies of M2e to one copy of foreign T helper cell epitopes. A high-yield reassortant H3N2 virus was generated by plasmid based reverse genetics. The efficacy of the novel H3N2 inactivated vaccines with or without M2e-MAP supplementation was evaluated in a mouse model. M2e-MAP conjugated vaccine induced strong antibody responses in mice. Complete protection against the heterologous swine H1N1 virus was observed in mice vaccinated with M2e-MAP combined vaccine. Moreover, this novel peptide confers protection against lethal challenge of A/Puerto Rico/8/34 (H1N1). Taken together, our results suggest the combined immunization of reassortant inactivated H3N2 vaccine and the novel M2e-MAP provided cross-protection against swine and human viruses and may serve as a promising approach for influenza vaccine development.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Peptides
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Réplication virale
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Poids
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Vaccins antigrippaux
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Vaccins synthétiques
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Répartition aléatoire
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Analyse de survie
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Infections à Orthomyxoviridae
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Déterminants antigéniques des lymphocytes T
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Modèles animaux de maladie humaine
Limites du sujet:
Animals
langue:
En
Texte intégral:
Journal of Veterinary Science
Année:
2016
Type:
Article