Effect of Transduced Tumor Necrosis Factor-alpha Gene Expression on Growth of Human Bladder Tumor Cell Lines / 대한비뇨기과학회지
Korean Journal of Urology
; : 1339-1344, 1996.
Article
Dans Ko
| WPRIM
| ID: wpr-117015
Responsable en Bibliothèque :
WPRO
ABSTRACT
OBJECTIVE:
To evaluate the effect of transduced tumor necrosis factor-a(TNF-a) gene expression on growth of human bladder tumor cell lines in vitro. MATERIALS ANDMETHODS:
The complete cDNA of TNF-a was introduced to three human bladder tumor cell lines(F-24, J-82, HT-1197) using a retroviral vector, a recombinant form of Molony murine leukemia virus with TNF-a and Neo gene and transfected cells were selected by exposure to neomycin analog G418. Gene transfer and expression were confirmed by polymerase chain reaction(PCR) and reverse transcription polymerase chain reaction(RT-PCR)-Southern blotting. Cell growth was measured by MTT assay Result is Successful gene transfer and expression were confirmed in all three cell bladder tumor lines. Growth of transfected cells were compared with parental cell lines and no differences were found in all three cell lines(p>0.05).CONCLUSION:
Expression of transduced TNF-t gene could not show any effect on growth of human bladder tumor cells in vitro.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Parents
/
Vessie urinaire
/
Tumeurs de la vessie urinaire
/
Néomycine
/
Thérapie génétique
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Zidovudine
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Expression des gènes
/
Lignée cellulaire
/
Facteur de nécrose tumorale alpha
/
ADN complémentaire
Limites du sujet:
Humans
langue:
Ko
Texte intégral:
Korean Journal of Urology
Année:
1996
Type:
Article