Rapid Detection and Identification of Wuchereria bancrofti, Brugia malayi, B. pahangi, and Dirofilaria immitis in Mosquito Vectors and Blood Samples by High Resolution Melting Real-Time PCR
The Korean Journal of Parasitology
; : 645-650, 2013.
Article
de En
| WPRIM
| ID: wpr-118761
Bibliothèque responsable:
WPRO
ABSTRACT
A simple, rapid, and high-throughput method for detection and identification of Wuchereria bancrofti, Brugia malayi, Brugia pahangi, and Dirofilaria immitis in mosquito vectors and blood samples was developed using a real-time PCR combined with high-resolution melting (HRM) analysis. Amplicons of the 4 filarial species were generated from 5S rRNA and spliced leader sequences by the real-time PCR and their melting temperatures were determined by the HRM method. Melting of amplicons from W. bancrofti, B. malayi, D. immitis, and B. pahangi peaked at 81.5+/-0.2degrees C, 79.0+/-0.3degrees C, 76.8+/-0.1degrees C, and 79.9+/-0.1degrees C, respectively. This assay is relatively cheap since it does not require synthesis of hybridization probes. Its sensitivity and specificity were 100%. It is a rapid and technically simple approach, and an important tool for population surveys as well as molecular xenomonitoring of parasites in vectors.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Parasitologie
/
Wuchereria bancrofti
/
Sang
/
Brugia
/
ARN ribosomique 5S
/
Sensibilité et spécificité
/
ARN des helminthes
/
Température de transition
/
Dirofilaria immitis
/
Réaction de polymérisation en chaine en temps réel
Type d'étude:
Diagnostic_studies
Limites du sujet:
Animals
/
Humans
/
Male
langue:
En
Texte intégral:
The Korean Journal of Parasitology
Année:
2013
Type:
Article