Evaluation of Antiangiogenic Effects of a New Synthetic Candidate Drug KR-31831 on Xenografted Ovarian Carcinoma Using Dynamic Contrast Enhanced MRI
Korean Journal of Radiology
; : 602-610, 2011.
Article
de En
| WPRIM
| ID: wpr-121835
Bibliothèque responsable:
WPRO
ABSTRACT
OBJECTIVE: The purpose of this research was to investigate the anti-angiogenic inhibitory effect of KR-31831, a newly developed anti-angiogenic agent, on an in vivo human ovarian carcinoma model using dynamic contrast-enhanced (DCE) MRI. MATERIALS AND METHODS: Xenografted ovarian tumors were established by subcutaneous injection of SKOV3 cells into mice. The mice were treated daily with KR-31831 at 50 mg/kg for 21 days. Tumor tissues were excised corresponding to the DCE-MRI sections for evaluation of MVD with CD31 immunohistochemistry. All in vivo MRIs were performed on a 7.0 Tesla micro-MRI System. DCE-MRI was acquired prior to initiating treatment with KR-31831 and again on days 3 and 21 after treatment. The permeability parameters (Ktrans, ve, and vp) were estimated using a pharmacokinetic model. RESULTS: Qualitatively, the Ktrans parametric mapping showed different changes before and after treatment with KR-31831 in the treatment group. For quantification of this change, the median of Ktrans values were compared before and after treatments in the control and KR-31831-treated groups. A non-parametric statistical test (Wilcoxon signed-rank test) showed decreasing Ktrans values on day 21 compared to days 0 and 3 in the KR-31831-treated group (p < 0.05), whereas there was no significant difference in the control group (p = 0.84). CONCLUSION: Our results suggest that DCE-MRI can be a useful tool by which to evaluate the anti-angiogenic effect of KR-31831 on a xenografted human ovarian carcinoma model.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Tumeurs de l'ovaire
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Benzopyranes
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Immunohistochimie
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Imagerie par résonance magnétique
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Produits de contraste
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Inhibiteurs de l'angiogenèse
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Lignée cellulaire tumorale
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Microvaisseaux
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Imidazoles
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Souris de lignée BALB C
Type d'étude:
Prognostic_studies
Limites du sujet:
Animals
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Female
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Humans
langue:
En
Texte intégral:
Korean Journal of Radiology
Année:
2011
Type:
Article