Prevalence of Dirofilaria immitis Infection in Stray Cats by Nested PCR in Korea
The Korean Journal of Parasitology
; : 691-694, 2014.
Article
de En
| WPRIM
| ID: wpr-124052
Bibliothèque responsable:
WPRO
ABSTRACT
The purpose of this study was to conduct a survey of Dirofilaria immitis infection among stray cats in Korea using nested PCR. We included 235 stray cats (121 females and 114 males) and evaluated each for the presence of feline heartworm infection. Blood samples were collected from 135 cats in Daejeon, 50 cats in Seoul, and 50 cats from Gyeonggi-do (Province). Of the 235 DNA samples, 14 (6.0%) were positive for D. immitis. The prevalence of infection in male cats (8/114, 7.0%) tended to be higher than that in female cats (6/121, 5.0%), but the difference was not statistically significant. In each location, 8, 2, and 4 cats were positive for infection, respectively, based on DNA testing. No significant differences in the prevalence were observed among the geographic regions, although the rate of infection was higher in Gyeonggi-do (8.0%) than Daejeon (5.9%) and Seoul (4.0%). We submitted 7 of the 14 D. immitis DNA-positive samples for sequencing analysis. All samples corresponded to partial D. immitis cytochrome c oxidase subunit I gene sequences with 99% homology to the D. immitis sequence deposited in GenBank (accession no. FN391553). To the best of our knowledge, this is the first survey using nested PCR to analyze the prevalence of D. immitis in stray cats in Korea.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Sang
/
Données de séquences moléculaires
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Maladies des chats
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Réaction de polymérisation en chaîne
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Prévalence
/
Similitude de séquences
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Analyse de séquence d'ADN
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Complexe IV de la chaîne respiratoire
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ADN des helminthes
/
Dirofilaria immitis
Type d'étude:
Prevalence_studies
/
Risk_factors_studies
Limites du sujet:
Animals
Pays comme sujet:
Asia
langue:
En
Texte intégral:
The Korean Journal of Parasitology
Année:
2014
Type:
Article