The relationship between cisplatin-induced apoptosis and p53, bcl-2 and bax expression in human lung cancer cells
The Korean Journal of Internal Medicine
; : 42-52, 1999.
Article
de En
| WPRIM
| ID: wpr-125514
Bibliothèque responsable:
WPRO
ABSTRACT
OBJECTIVES: Given the roles of bcl-2, bax and p53 in apoptosis, we investigated the effect of their expression on the response to cisplatin in order to understand the molecular events of cisplatin-resistance in lung cancers. METHODS: Three parental human lung cancer cell lines (PC9, PC14 and H69) and their in vitro selected cisplatin-resistant sublines were examined. Cells treated with cisplatin were processed for acridine orange and ethidium bromide staining and DNA gel electrophoresis for the morphologic detection of apoptosis. The endogenous levels of bcl-2, bax and p53 protein expression in lung cancer cells were assessed by Western blot analysis and DNA of polymerase chain reaction-amplified exon 5 to 8 of p53 gene was directly sequenced. RESULTS: H69, which had bcl-2 expression, p53 mutation and decreased expression of p53 and bax, was relatively resistant to cisplatin and delayed and reduced apoptosis. Although apoptosis was markedly reduced in cisplatin-resistant sublines compared to their parental cells, there were no significant differences in the expression of p53, bcl-2 and bax. CONCLUSIONS: Cisplatin-resistance was associated with the reduced cellular susceptibility to apoptosis. Cancer cells with the natural expression of bcl-2 and p53 mutation may be more resistant to cisplatin and less susceptible to apoptosis.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Résistance aux substances
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Cellules cancéreuses en culture
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Expression des gènes
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Gènes p53
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Protéines proto-oncogènes
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Cisplatine
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Apoptose
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Gènes bcl-2
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Tumeurs du poumon
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Antinéoplasiques
Limites du sujet:
Humans
langue:
En
Texte intégral:
The Korean Journal of Internal Medicine
Année:
1999
Type:
Article