Sequencing of p53 Gene Mutations in Primary Breast Cancer Tissues / 대한암학회지
Journal of the Korean Cancer Association
; : 663-669, 1999.
Article
Dans Ko
| WPRIM
| ID: wpr-126876
Responsable en Bibliothèque :
WPRO
ABSTRACT
PURPOSE:
The etiology of breast cancer involves very complex factors such as genetic, hormonal, and dietary. The peak age of Korean breast cancer is much earlier, about ten years, than those of western countries. The role of p53 gene on the carcinogenesis has been studied since 1991. This study was designed for the evaluation of genetic factor by determining p53 gene mutations in Korean breast cancer. MATERIALS ANDMETHODS:
Mutation screening on p53 tumor suppressor gene was examined with PCR-SSCP and nucleotide sequencing technique from the genomic DNA extracted from the 27 fresh-frozen breast cancer tissues.RESULTS:
Mutations in p53 gene exon 5-7 were identified in 2 of 27 cases (7%). One had a missense mutation substituted gcg with ggg at codon 159, exon 5, and the other had a point mutation substituted tcc serine to tGc cysteine at codon 241, exon 7.CONCLUSION:
Point mutation of p53 gene in breast cancer seems to be the major defect found in Korean patients. It is necessary to perform further study in mutation of other exon 2, 4, 8, 9, and 11 of p53 gene to compare the genetic backgrounds of Korean breast cancer with those of westerns.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Sérine
/
Région mammaire
/
Tumeurs du sein
/
Codon
/
ADN
/
Dépistage de masse
/
Exons
/
Gènes p53
/
Gènes suppresseurs de tumeur
/
Mutation ponctuelle
Type d'étude:
Prognostic_studies
/
Screening_studies
Limites du sujet:
Humans
langue:
Ko
Texte intégral:
Journal of the Korean Cancer Association
Année:
1999
Type:
Article