A simple rat model of in situ reversible obstructive jaundice in situ reversible obstructive jaundice model
Annals of Surgical Treatment and Research
; : 389-395, 2017.
Article
de En
| WPRIM
| ID: wpr-131728
Bibliothèque responsable:
WPRO
ABSTRACT
PURPOSE: To develop a simple and reliable rat model of in situ reversible obstructive jaundice with low morbidity and mortality rates. METHODS: Rats were divided into 4 groups with 8 rats each: the sham-operated (SH) group only underwent laparotomy, the control internal drainage (ID-C) group underwent choledochoduodenostomy, the new internal drainage (ID-N) group and the long-term internal drainage (ID-L) group underwent choledochocholedochostomy. Common bile duct ligation was performed in all the drainage groups 7 days before reversal procedures. All rats were sacrificed for samples 7 days after the last operation except rats of the ID-L group that survived 28 days before sacrifice. Body weight, liver function, histopathological changes, morbidity and mortality were assessed. RESULTS: One rat died and 2 rats had complications with tube blockage in the ID-C group. No death or complications occurred in the ID-N and ID-L groups. The drainage tube remained patent in the long-term observation ID-L group. Body weight showed no significant difference between the ID-C and ID-N groups after 7 days drainage. Liver function was not fully recovered in the ID-C and ID-N groups after 7 days drainage, but statistical differences were only observed in the ID-C group compared with the SH and ID-L groups. Periportal inflammation and bile duct proliferation showed severer in the ID-C group than in the ID-N group. CONCLUSION: The present study provided an efficient, simple, and reliable rat model that is especially suitable for long-term or consecutive studies of reversible obstructive jaundice.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Conduits biliaires
/
Poids
/
Cholédocostomie
/
Drainage
/
Mortalité
/
Conduit cholédoque
/
Modèles animaux
/
Ictère rétentionnel
/
Inflammation
/
Laparotomie
Type d'étude:
Prognostic_studies
Limites du sujet:
Animals
langue:
En
Texte intégral:
Annals of Surgical Treatment and Research
Année:
2017
Type:
Article