Expression of Glucagon-Like Peptide 1 Receptor during Osteogenic Differentiation of Adipose-Derived Stem Cells
Endocrinology and Metabolism
; : 567-573, 2014.
Article
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| WPRIM
| ID: wpr-14695
Responsable en Bibliothèque :
WPRO
ABSTRACT
BACKGROUND:
Glucagon-like peptide 1 (GLP-1), an incretin hormone well known for its glucose-lowering effect, was recently reported to exert an anabolic effect on bone. Although the exact mechanism is not known, it likely involves the GLP-1 receptor (GLP-1R), which is expressed in some osteoblastic cell lines. Adipose-derived stem cells (ADSCs) have mesenchymal stem cell-specific characteristics, including osteoblastic differentiation potential. We evaluated the expression of GLP-1R during osteogenic differentiation of ADSCs.METHODS:
ADSCs were isolated from subcutaneous adipose tissue obtained from three male donors during plastic surgery and were subjected to osteogenic induction. Mineralization was assessed by Alizarin Red staining on day 21. Expression of alkaline phosphatase (ALP), osteocalcin (OC), and GLP-1R was measured by real-time polymerase chain reaction in triplicate for each patient on days 0, 7, 14, and 21. Target mRNA expression levels were normalized to that of beta-actin.RESULTS:
ADSCs were fibroblast-like in morphology, adhered to plastic, and had multipotent differentiation potential, as assessed using specific antigen markers. The osteogenic markers ALP and OC were notably upregulated at 21 days. Osteogenic differentiation resulted in a time-dependent increase in the expression of GLP-1R (P=0.013).CONCLUSION:
We demonstrated upregulation of GLP-1R gene expression during osteogenic differentiation of ADSCs. This finding suggests that GLP-1 may induce osteogenic differentiation in bone tissue.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Ostéoblastes
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Ostéogenèse
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Cellules souches
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Chirurgie plastique
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Donneurs de tissus
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Os et tissu osseux
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ARN messager
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Expression des gènes
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Ostéocalcine
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Régulation positive
Limites du sujet:
Humans
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Male
langue:
En
Texte intégral:
Endocrinology and Metabolism
Année:
2014
Type:
Article