Swiprosin-1 Expression Is Up-Regulated through Protein Kinase C-theta and NF-kappaB Pathway in T Cells
Immune Network
; : 55-62, 2013.
Article
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| WPRIM
| ID: wpr-147332
Responsable en Bibliothèque :
WPRO
ABSTRACT
Swiprosin-1 exhibits the highest expression in CD8+ T cells and immature B cells and has been proposed to play a role in lymphocyte biology through actin remodeling. However, regulation of swiprosin-1 gene expression is poorly understood. Here we report that swiprosin-1 is up-regulated in T cells by PKC pathway. Targeted inhibition of the specific protein kinase C (PKC) isotypes by siRNA revealed that PKC-theta is involved in the expression of swiprosin-1 in the human T cells. In contrast, down-regulation of swiprosin-1 by A23187 or ionomycin suggests that calcium-signaling plays a negative role. Interestingly, swiprosin-1 expression is only reduced by treatment with NF-kappaB inhibitors but not by NF-AT inhibitor, suggesting that the NF-kappaB pathway is critical for regulation of swiprosin-1 expression. Collectively, these results suggest that swiprosin-1 is a PKC-theta-inducible gene and that it may modulate the late phase of T cell activation after antigen challenge.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Protein kinases
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Biologie
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Protéine kinase C
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Lymphocytes
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Lymphocytes T
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Régulation négative
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Expression des gènes
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Ionomycine
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Facteur de transcription NF-kappa B
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A-23187
Limites du sujet:
Humans
langue:
En
Texte intégral:
Immune Network
Année:
2013
Type:
Article