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Caveolin-1 is involved in high glucose accelerated human glomerular mesangial cell senescence
Article de En | WPRIM | ID: wpr-151261
Bibliothèque responsable: WPRO
ABSTRACT
BACKGROUND/AIMS:: We demonstrated the role of caveolin-1 involved in high glucose (HG)-induced glomerular mesangial cells (GMCs) senescence. METHODS:: HG was used to stimulate GMCs. The telomere lengths were analyzed by Southern blot. β-Galactosidase staining was determined. The expressions of caveolin-1 and P53 proteins were determined by Western blot. RESULTS:: Treatment with high concentrations of glucose induced GMC senescence accompanied by shortened telomere length and increase of β-galactosidase staining as well as P53 protein, which was abrogated after application of caveolin-1-siRNA. CONCLUSIONS:: This study proved that HG induced cell senescence in GMCs. The caveolin-1 is involved in HG-induced mesangial cell senescence, and blocking caveolin-1 significantly reduced cell senescence. The effect of caveolin-1 is mediated by P53 pathway.
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Texte intégral: 1 Indice: WPRIM Sujet Principal: Vieillissement / Technique de Western / Technique de Southern / Télomère / Vieillissement de la cellule / Cellules mésangiales / Cavéoline-1 / Glucose Limites du sujet: Humans langue: En Texte intégral: The Korean Journal of Internal Medicine Année: 2017 Type: Article
Texte intégral: 1 Indice: WPRIM Sujet Principal: Vieillissement / Technique de Western / Technique de Southern / Télomère / Vieillissement de la cellule / Cellules mésangiales / Cavéoline-1 / Glucose Limites du sujet: Humans langue: En Texte intégral: The Korean Journal of Internal Medicine Année: 2017 Type: Article