Synergistic Effect of Parthenolide in Combination with 5-Fluorouracil in SW480 Cells
Intestinal Research
; : 357-364, 2012.
Article
de Ko
| WPRIM
| ID: wpr-154834
Bibliothèque responsable:
WPRO
ABSTRACT
BACKGROUND/AIMS: Parthenolide (PT) is responsible for the bioactivities of Feverfew. Besides its potent anti-inflammatory effect, this compound has recently been reported to induce apoptosis in cancer cells. Unfortunately, many of the therapies that use 5-fluorouracil (5-FU) alone or in combination with other agents are likely to become ineffective due to drug resistance. In the present study, we investigate the antitumor effect of PT combined with 5-FU on colorectal cancer cells. METHODS: SW480 cell was employed as a representative of human colorectal carcinoma (CRC) cells. We performed MTT, annexin-V assay, and Hoechst 33258 staining to measure the synergistic effect. Western blotting was used to demonstrate apoptotic pathway. RESULTS: Our result demonstrated that PT inhibited the viability of colorectal cancer cells and had synergistic anti-proliferation in combination with 5-FU. After combined treatment of 5-FU and PT, enhanced apoptotic cell death is observed using annexin-V FITC assay and it was revealed by the condensed chromatin and fragmented DNA. Compared with 5-FU or PT alone, the apoptosis of colorectal cancer cells treated with PT and 5-FU enhanced the activation of caspase-8, caspase-3. CONCLUSIONS: Combined treatment with PT may offer an efficacious strategy to overcome 5-FU resistance in certain CRC cells.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Sesquiterpènes
/
ADN
/
Résistance aux substances
/
Chromatine
/
Tumeurs colorectales
/
Technique de Western
/
Fluorescéine-5-isothiocyanate
/
Mort cellulaire
/
Apoptose
/
Tanacetum parthenium
Limites du sujet:
Humans
langue:
Ko
Texte intégral:
Intestinal Research
Année:
2012
Type:
Article