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Synergistic Effect of Parthenolide in Combination with 5-Fluorouracil in SW480 Cells
Intestinal Research ; : 357-364, 2012.
Article de Ko | WPRIM | ID: wpr-154834
Bibliothèque responsable: WPRO
ABSTRACT
BACKGROUND/AIMS: Parthenolide (PT) is responsible for the bioactivities of Feverfew. Besides its potent anti-inflammatory effect, this compound has recently been reported to induce apoptosis in cancer cells. Unfortunately, many of the therapies that use 5-fluorouracil (5-FU) alone or in combination with other agents are likely to become ineffective due to drug resistance. In the present study, we investigate the antitumor effect of PT combined with 5-FU on colorectal cancer cells. METHODS: SW480 cell was employed as a representative of human colorectal carcinoma (CRC) cells. We performed MTT, annexin-V assay, and Hoechst 33258 staining to measure the synergistic effect. Western blotting was used to demonstrate apoptotic pathway. RESULTS: Our result demonstrated that PT inhibited the viability of colorectal cancer cells and had synergistic anti-proliferation in combination with 5-FU. After combined treatment of 5-FU and PT, enhanced apoptotic cell death is observed using annexin-V FITC assay and it was revealed by the condensed chromatin and fragmented DNA. Compared with 5-FU or PT alone, the apoptosis of colorectal cancer cells treated with PT and 5-FU enhanced the activation of caspase-8, caspase-3. CONCLUSIONS: Combined treatment with PT may offer an efficacious strategy to overcome 5-FU resistance in certain CRC cells.
Sujet(s)
Mots clés
Texte intégral: 1 Indice: WPRIM Sujet Principal: Sesquiterpènes / ADN / Résistance aux substances / Chromatine / Tumeurs colorectales / Technique de Western / Fluorescéine-5-isothiocyanate / Mort cellulaire / Apoptose / Tanacetum parthenium Limites du sujet: Humans langue: Ko Texte intégral: Intestinal Research Année: 2012 Type: Article
Texte intégral: 1 Indice: WPRIM Sujet Principal: Sesquiterpènes / ADN / Résistance aux substances / Chromatine / Tumeurs colorectales / Technique de Western / Fluorescéine-5-isothiocyanate / Mort cellulaire / Apoptose / Tanacetum parthenium Limites du sujet: Humans langue: Ko Texte intégral: Intestinal Research Année: 2012 Type: Article