IL-6 gene promoter polymorphisms in Korean generalized aggressive periodontitis / 대한치주과학회지
The Journal of the Korean Academy of Periodontology
; : 579-588, 2008.
Article
de Ko
| WPRIM
| ID: wpr-157284
Bibliothèque responsable:
WPRO
ABSTRACT
PURPOSE: The purpose of this study was to investigate the association of generalized aggressive periodontitis with IL-6 promoter gene single nucleotide polymorphisms(SNP). MATERIAL AND METHODS: The study population consisted of 52 generalized aggressive periodontitis patients(GAP) and 30 periodontally healthy control subjects, who were systemically healthy non-smokers. Genomic DNA was obtained from buccal swab. The IL-6 promotor SNP at the positions of -597, -572, and -174 were genotyped by amplifying the polymorphic region using polymerase chain reaction(PCR), restriction enzyme digestion and gel electrophoresis. RESULT: The genotype distributions for G/G, G/A and A/A genotypes of IL-6 -597 were 30.8%, 40.4%, and 28.8% in the GAP group and 53.3%, 40%, and 6.7% in the control group and were statistically different between 2 groups(p<0.05). Allele 2 frequency of IL-6 -597 were significantly higher in the GAP group than the control group(p<0.01). At the position of IL-6 -572, the distribution for C/C, C/G and G/G genotypes were 23.1%, 55.8% and 21.2% in the GAP group and 20%, 33.3%, and 46.7% in the control group. In female subjects, the genotype distribution were significantly different between 2 groups (p<0.01). In male subjects, allele 2 frequency of IL-6-572 was significantly lower in the GAP group than the control group(p<0.05). The genotype distribution of IL-6 -174 in the GAP group were 96.2%, 3.8% for G/G, G/C genotypes whereas only the G/G genotype was detected in the control group. CONCLUSION: In conclusion, significant associations were found in IL-6 gene promoter(-597, -572) polymorphisms and generalized aggressive periodontitis. Further cohort study will be necessary in larger population.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Parodontite agressive
/
ADN
/
Études de cohortes
/
Interleukine-6
/
Digestion
/
Allèles
/
Génotype
Type d'étude:
Etiology_studies
/
Incidence_studies
/
Observational_studies
/
Risk_factors_studies
Limites du sujet:
Female
/
Humans
/
Male
langue:
Ko
Texte intégral:
The Journal of the Korean Academy of Periodontology
Année:
2008
Type:
Article