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Effect of Prunetin on TNF-alpha-Induced MUC5AC Mucin Gene Expression, Production, Degradation of IkappaB and Translocation of NF-kappaB p65 in Human Airway Epithelial Cells / 결핵및호흡기질환
Article de En | WPRIM | ID: wpr-157866
Bibliothèque responsable: WPRO
ABSTRACT
BACKGROUND: We investigated whether prunetin significantly affects tumor necrosis factor-alpha (TNF-alpha)-induced MUC5AC mucin gene expression, production, inhibitory kappa B (IkappaB) degradation and nuclear factor kappa B (NF-kappaB) p65 translocation in human airway epithelial cells. METHODS: Confluent NCI-H292 cells were pretreated with prunetin for 30 minutes and then stimulated with TNF-alpha for 24 hours or the indicated periods. MUC5AC mucin gene expression and mucin protein production were measured by reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. The effect of prunetin on TNF-alpha-induced degradation of IkappaB and translocation of NF-kappaB p65 was investigated by western blot analysis. RESULTS: We found that incubation of NCI-H292 cells with prunetin significantly inhibited mucin production and down-regulated the MUC5AC gene expression induced by TNF-alpha. Prunetin inhibited TNF-alpha-induced degradation of IkappaB and translocation of NF-kappaB p65. CONCLUSION: This result suggests that prunetin inhibits the NF-kappaB signaling pathway, which may explain its role in the inhibition of MUC5AC mucin gene expression and production regulated by the NF-kappaB signaling pathway.
Sujet(s)
Mots clés
Texte intégral: 1 Indice: WPRIM Sujet Principal: Test ELISA / Expression des gènes / Technique de Western / Réaction de polymérisation en chaîne / Facteur de transcription NF-kappa B / Facteur de nécrose tumorale alpha / Transcription inverse / Cellules épithéliales / Mucine-5AC / Isoflavones Limites du sujet: Humans langue: En Texte intégral: Tuberculosis and Respiratory Diseases Année: 2013 Type: Article
Texte intégral: 1 Indice: WPRIM Sujet Principal: Test ELISA / Expression des gènes / Technique de Western / Réaction de polymérisation en chaîne / Facteur de transcription NF-kappa B / Facteur de nécrose tumorale alpha / Transcription inverse / Cellules épithéliales / Mucine-5AC / Isoflavones Limites du sujet: Humans langue: En Texte intégral: Tuberculosis and Respiratory Diseases Année: 2013 Type: Article