A Quantitative Study of Airway Changes on Micro-CT in a Mouse Asthma Model: Comparison With Histopathological Findings
Allergy, Asthma & Immunology Research
; : 75-82, 2014.
Article
Dans En
| WPRIM
| ID: wpr-164117
Responsable en Bibliothèque :
WPRO
ABSTRACT
PURPOSE:
To evaluate airway changes in ovalbumin-induced asthmatic mice in terms of postmortem micro-CT images and pathological findings.METHODS:
Asthma was induced in mice by intraperitoneal injection and nasal instillation of ovalbumin aluminium hydroxide into mice (experimental group, n=6), and another group of mice received intraperitoneal injection and nasal instillation of distilled phosphate-buffered saline (control group, n=6). Bronchial lumen area was measured in the main bronchial lumen of the distal third bronchial branch level (6 parts per each mouse) on axial scans of Micro-CT, using a Lucion's smart pen (semi-automated) and a curve pen (manual). Bronchial wall thickness was obtained in 4 sections (2 levels on either side) after the third bronchial branch by measuring the diameter which was perpendicular to the longitudinal axis of the main bronchus on curved Multi-planar reconstruction (MPR) images. Histologic slides were obtained from the lesion that was matched with its CT images, and bronchial wall thicknesses were determined.RESULTS:
The mean bronchial lumen area was 0.196+/-0.072 mm2 in the experimental group and 0.243+/-0.116 mm2 in the control group; the difference was significant. Bronchial wall thickness on micro-CT images (mean, 0.119+/-0.01 vs. 0.108+/-0.013 mm) and in pathological specimens (mean, 0.066+/-0.011 vs. 0.041+/-0.009 mm) were thicker in the experimental group than in the control group; bronchial wall thickness on micro-CT images correlated well with pathological thickness (for the experimental group, r=0.712; for the control group, r=0.46). The thick bronchial wall in the experimental group demonstrated submucosal hypertrophy along with goblet cell hyperplasia and smooth muscle hyperplasia.CONCLUSIONS:
The results of this study suggest that asthma may induce thickening of bronchial wall and narrowing of the lumen area on micro-CT images and that these results may significantly correlate with pathological findings.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Asthme
/
Axis
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Bronches
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Études cas-témoins
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Ovalbumine
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Cellules caliciformes
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Études d'évaluation comme sujet
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Microtomographie aux rayons X
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Remodelage des voies aériennes
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Hyperplasie
Type d'étude:
Diagnostic_studies
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Evaluation_studies
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Observational_studies
/
Prognostic_studies
Limites du sujet:
Animals
langue:
En
Texte intégral:
Allergy, Asthma & Immunology Research
Année:
2014
Type:
Article