Real-Time Quaking-Induced Conversion Analysis for the Diagnosis of Sporadic Creutzfeldt-Jakob Disease in Korea
Journal of Clinical Neurology
; : 101-106, 2016.
Article
Dans En
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| ID: wpr-166853
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WPRO
ABSTRACT
BACKGROUND AND PURPOSE:
The level of 14-3-3 protein in the cerebrospinal fluid (CSF) is increased in Creutzfeldt-Jakob disease (CJD) patients, which has led to it being used as a clinical biomarker for the ante-mortem diagnosis of human prion diseases. However, the specificity of the 14-3-3 protein is less reliable for CJD diagnosis. Newly developed assays including real-time quaking-induced conversion (RT-QuIC) have made it possible to detect the PrPSc-like abnormal prion isoform with a high sensitivity in animal and human specimens that might contain a minute amount of PrP(Sc) due to in vitro prion replication.METHODS:
This study applied a highly sensitive RT-QuIC assay using recombinant human PrP to detect PrP(Sc) in the CSF of 81 patients with sporadic CJD (sCJD) in Korea.RESULTS:
RT-QuIC analysis of the CSF samples based on the expression levels of 14-3-3 and total tau proteins revealed positivity in 62 of 81 sCJD patients (sensitivity of 76.5%) but no positive results in the 100 non-CJD patients.CONCLUSIONS:
The sensitivity of the RT-QuIC in this study was similar to that in some previous reports, and the specificity of RT-QuIC was higher than that of 14-3-3 in CSF, suggesting that RT-QuIC analysis can complement the weakness of the specificity of 14-3-3 for the diagnosis of sCJD. These results indicate that RT-QuIC might be very useful for the rapid and specific diagnosis of sCJD and provide a practical novel method for the ante-mortem diagnosis of human prion diseases.
Texte intégral:
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Indice:
WPRIM
Sujet Principal:
Protéines du système du complément
/
Liquide cérébrospinal
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Maladie de Creutzfeldt-Jakob
/
Sensibilité et spécificité
/
Protéines tau
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Maladies à prions
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Protéines 14-3-3
/
Diagnostic
/
Corée
Type d'étude:
Diagnostic_studies
Limites du sujet:
Animals
/
Humans
Pays comme sujet:
Asia
langue:
En
Texte intégral:
Journal of Clinical Neurology
Année:
2016
Type:
Article