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Relationship Between the Extent of Chromosomal Losses and the Pattern of CpG Methylation in Gastric Carcinomas
Article de En | WPRIM | ID: wpr-176546
Bibliothèque responsable: WPRO
ABSTRACT
The extent of unilateral chromosomal losses and the presence of microsatellite instability (MSI) have been classified into high-risk (high- and baseline-level loss) and low-risk (low-level loss and MSI) stem-line genotypes in gastric carcinomas. A unilateral genome-dosage reduction might stimulate compensation mechanism, which maintains the genomic dosage via CpG hypomethylation. A total of 120 tumor sites from 40 gastric carcinomas were examined by chromosomal loss analysis using 40 microsatellite markers on 8 chromosomes and methylation analysis in the 13 CpG (island/non-island) regions near the 10 genes using the bisulfite-modified DNAs. The high-level-loss tumor (four or more losses) showed a tendency toward unmethylation in the Maspin, CAGE, MAGE-A2 and RABGEF1 genes, and the other microsatellite-genotype (three or fewer losses and MSI) toward methylation in the p16, hMLH1, RASSF1A, and Cyclin D2 genes (p<0.05). The non-island CpGs of the p16 and hMLH1 genes were hypomethylated in the high-level-loss and hypermethylated in the non-high-level-loss sites (p<0.05). Consequently, hypomethylation changes were related to a high-level loss, whereas the hypermethylation changes were accompanied by a baseline-level loss, a low-level loss, or a MSI. This indicates that hypomethylation compensates the chromosomal losses in the process of tumor progression.
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Texte intégral: 1 Indice: WPRIM Sujet Principal: Polymorphisme génétique / Tumeurs de l'estomac / Analyse de mutations d'ADN / Dépistage génétique / Incidence / Facteurs de risque / Aberrations des chromosomes / Cartographie chromosomique / Appréciation des risques / Répétitions microsatellites Type d'étude: Etiology_studies / Incidence_studies / Prognostic_studies / Risk_factors_studies Limites du sujet: Humans Pays comme sujet: Asia / Europa langue: En Texte intégral: Journal of Korean Medical Science Année: 2005 Type: Article
Texte intégral: 1 Indice: WPRIM Sujet Principal: Polymorphisme génétique / Tumeurs de l'estomac / Analyse de mutations d'ADN / Dépistage génétique / Incidence / Facteurs de risque / Aberrations des chromosomes / Cartographie chromosomique / Appréciation des risques / Répétitions microsatellites Type d'étude: Etiology_studies / Incidence_studies / Prognostic_studies / Risk_factors_studies Limites du sujet: Humans Pays comme sujet: Asia / Europa langue: En Texte intégral: Journal of Korean Medical Science Année: 2005 Type: Article