Mutation of the Chk1 Gene in Gastric Cancers with Microsatellite Instability
Journal of the Korean Gastric Cancer Association
; : 260-265, 2005.
Article
Dans Ko
| WPRIM
| ID: wpr-189860
Responsable en Bibliothèque :
WPRO
ABSTRACT
PURPOSE:
The protein kinase Chk1 is required for cell cycle arrest in response to DNA damage and is shown to play an important role in the G2/M checkpoint. The aim of this study was to investigate the relationship between microsatellite instability and frameshift mutation of the Chk1 gene in gastric cancers. MATERIALS ANDMETHODS:
The microsatellite instability was analyzed in 95 primary gastric carcinomas by using microdissection and 6 microsatellite markers. We also performed single strand conformational polymorphism and sequencing to detect frameshift mutation of the Chk1 gene.RESULTS:
We found positive microsatellite instability in 19 (20%) of the 95 gastric cancers, 13 high- and 6 low-frequency microsatellite instability cases. The frameshift mutation of Chk1, which resulted in a truncated Chk1 protein, was detected in two high-frequency microsatellite instability cases.CONCLUSION:
These data suggest that the microsatellite instability may contribute to the development of gastric carcinomas through inactivation of Chk1.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Protein kinases
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Tumeurs de l'estomac
/
Altération de l'ADN
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Cycle cellulaire
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Mutation avec décalage du cadre de lecture
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Répétitions microsatellites
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Microdissection
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Instabilité des microsatellites
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Points de contrôle du cycle cellulaire
langue:
Ko
Texte intégral:
Journal of the Korean Gastric Cancer Association
Année:
2005
Type:
Article