Chlorogenic Acid Decreases Retinal Vascular Hyperpermeability in Diabetic Rat Model
Journal of Korean Medical Science
; : 608-613, 2013.
Article
de En
| WPRIM
| ID: wpr-194140
Bibliothèque responsable:
WPRO
ABSTRACT
To evaluate the effect of chlorogenic acid (CGA), a polyphenol abundant in coffee, on retinal vascular leakage in the rat model of diabetic retinopathy, Sprague-Dawley rats were divided into four groups: controls, streptozotocin-induced diabetic rats, and diabetic rats treated with 10 and 20 mg/kg chlorogenic acid intraperitoneally daily for 14 days, respectively. Blood-retinal barrier (BRB) breakdown was evaluated using FITC-dextran. Vascular endothelial growth factor (VEGF) distribution and expression level was evaluated with immunohistochemistry and Western blot analysis. Expression of tight junction proteins, occludin and claudin-5, and zonula occludens protein, ZO-1 was also evaluated with immunohistochemistry and Western blot analysis. BRB breakdown and increased vascular leakage was found in diabetic rats, with increased VEGF expression and down-regulation of occludin, claudin-5, and ZO-1. CGA treatment effectively preserved the expression of occludin, and decreased VEGF levels, leading to less BRB breakdown and less vascular leakage. CGA may have a preventive role in BRB breakdown in diabetic retinopathy by preserving tight junction protein levels and low VEGF levels.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Rétine
/
Barrière hématorétinienne
/
Régulation négative
/
Acide chlorogénique
/
Dextrane
/
Fluorescéine-5-isothiocyanate
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Rat Sprague-Dawley
/
Facteur de croissance endothéliale vasculaire de type A
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Diabète expérimental
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Rétinopathie diabétique
Type d'étude:
Prognostic_studies
Limites du sujet:
Animals
langue:
En
Texte intégral:
Journal of Korean Medical Science
Année:
2013
Type:
Article