NAD(P)H: Quinone Oxidoreductase 1 and NRH:Quinone Oxidoreductase 2 Polymorphisms in Papillary Thyroid Microcarcinoma: Correlation with Phenotype
Yonsei Medical Journal
; : 1158-1167, 2013.
Article
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| WPRIM
| ID: wpr-198360
Bibliothèque responsable:
WPRO
ABSTRACT
PURPOSE: NAD(P)H:Quinone Oxidoreductase 1 (NQO1) C609T missense variant (NQO1*2) and 29 basepair (bp)-insertion/deletion (I29/D) polymorphism of the NRH:Quinone Oxidoreductase 2 (NQO2) gene promoter have been proposed as predictive and prognostic factors for cancer development and progression. The purpose of this study is to investigate the relationship between NQO1/NQO2 genotype and clinico-pathological features of papillary thyroid microcarcinoma (PTMC). MATERIALS AND METHODS: Genomic DNA was isolated from 243 patients; and clinical data were retrospectively analyzed. NQO1*2 and tri-allelic polymorphism of NQO2 were investigated by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis. RESULTS: PTMC with NQO1*2 frequently exhibited extra-thyroidal extension as compared to PTMC with wild-type NQO1 (p=0.039). There was a significant relationship between I29/I29 homozygosity of NQO2 and lymph node metastasis (p=0.042). Multivariate analysis showed that the I29/I29 genotype was associated with an increased risk of lymph node metastasis (OR, 2.24; 95% CI, 1.10-4.56; p=0.026). CONCLUSION: NQO1*2 and I29 allele of the NQO2 are associated with aggressive clinical phenotypes of PTMC, and the I29 allele represents a putative prognostic marker for PTMC.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Phénotype
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Polymorphisme génétique
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Pronostic
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Tumeurs de la thyroïde
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Analyse de mutations d'ADN
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Immunohistochimie
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Carcinome papillaire
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Analyse multifactorielle
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Études rétrospectives
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Mutagenèse par insertion
Type d'étude:
Observational_studies
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Prognostic_studies
Limites du sujet:
Adult
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Female
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Humans
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Male
langue:
En
Texte intégral:
Yonsei Medical Journal
Année:
2013
Type:
Article